Proteomics reveals the changes that contribute to Fusarium head blight resistance in wheat
Proteomics reveals the changes that contribute to Fusarium head blight resistance in wheat
复制标题
蛋白质组学揭示了小麦抗赤霉病的变化
DOI：
10.1094/phyto-05-20-0171-r
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发表时间：
2020
期刊：
影响因子：
3.2
通讯作者：
Shanshan Wen
中科院分区：
文献类型：
--
作者：
Mingming Yang;Xianguo Wang;Jian Dong;Wanchun Zhao;Tariq Alam;Linda S Thomashow;David M. Weller;Xiang Gao;Sachin Rustgi;Shanshan Wen
小麦赤霉病是小麦的一种毁灭性病害，由于产生真菌毒素而导致产量损失和品质下降。本研究采用iTRAQ（isobaric tags for relative and absolute quantification）标记的质谱技术对小麦品种西农538和周麦18的蛋白质组进行了分析，并以赤霉病抗性水平为对照，探讨了小麦赤霉病抗性的分子机制。接种禾谷镰刀菌48 h后，两个品种共鉴定出13，669个蛋白。其中，西农538（抗病）中有2,505个特异蛋白，周麦18（感病）中有887个特异蛋白。基因本体富集分析表明，大多数差异积累的蛋白质（DAP）从两个品种被分配到以下类别：代谢过程，单生物过程，细胞过程，和对刺激的反应。京都基因百科全书和基因组分析表明，西农538与周麦18相比，鉴定出更多属于不同代谢途径的蛋白质。具体而言，来自FHB抗性栽培品种西农538的DAP占据与植物-病原体相互作用相关的代谢途径的类别。这些DAPs可能在西农538的防御反应中起重要作用。来自两种基因型的DAP被分配到除染色体6 B之外的所有小麦染色体，其中约30%定位到小麦染色体2B、3B、5 B和5D。20个单核苷酸多态性标记，侧翼DAP染色体1B，3B，5 B和6A，重叠的位置，早期映射FHB抗性数量性状基因座。这些数据提供了证据，在小麦FHB抗性反应的早期阶段的几个DAP的参与，然而，候选蛋白质的进一步功能表征是必要的。
Fusarium head blight (FHB) is a devastating disease of wheat, causing yield losses and quality reduction as a result of mycotoxin production. In this study, iTRAQ (isobaric tags for relative and absolute quantification)-labeling-based mass spectrometry was employed to characterize the proteome in wheat cultivars Xinong 538 and Zhoumai 18 with contrasting levels of FHB resistance as a means to elucidate the molecular mechanisms contributing to FHB resistance. A total of 13,669 proteins were identified in the two cultivars 48 h afterFusarium graminearuminoculation. Among these, 2,505 unique proteins exclusively accumulated in Xinong 538 (resistant) and 887 proteins in Zhoumai 18 (susceptible). Gene Ontology enrichment analysis showed that most differentially accumulated proteins (DAPs) from both cultivars were assigned to the following categories: metabolic process, single-organism process, cellular process, and response to stimulus. Kyoto Encyclopedia of Genes and Genomes analysis showed that a greater number of proteins belonging to different metabolic pathways were identified in Xinong 538 compared with Zhoumai 18. Specifically, DAPs from the FHB-resistant cultivar Xinong 538 populated categories of metabolic pathways related to plant–pathogen interaction. These DAPs might play a critical role in defense responses exhibited by Xinong 538. DAPs from both genotypes were assigned to all wheat chromosomes except chromosome 6B, with approximately 30% mapping to wheat chromosomes 2B, 3B, 5B, and 5D. Twenty single nucleotide polymorphism markers, flanking DAPs on chromosomes 1B, 3B, 5B, and 6A, overlapped with the location of earlier mapped FHB-resistance quantitative trait loci. The data provide evidence for the involvement of several DAPs in the early stages of the FHB-resistance response in wheat; however, further functional characterization of candidate proteins is warranted.