A plant vacuolar protease, VPE, mediates virus-induced hypersensitive cell death
A plant vacuolar protease, VPE, mediates virus-induced hypersensitive cell death
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DOI：
10.1126/science.1099859
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发表时间：
2004-08-06
期刊：
影响因子：
56.9
通讯作者：
Hara-Nishimura, I
中科院分区：
文献类型：
--
作者：
Hatsugai, N;Kuroyanagi, M;Hara-Nishimura, I
动物的程序性细胞死亡（PCD）取决于caspase蛋白酶的活性。植物也表现出PCD，例如作为对病原体的反应，尽管植物半胱天冬酶仍然难以捉摸。在这里，我们表明液泡加工酶（VPE）是病毒诱导的涉及PCD的超敏反应所必需的蛋白酶。VPE缺乏可预防病毒诱导的烟草植株超敏细胞死亡。VPE在结构上与caspase无关，尽管VPE具有caspase-1活性。因此，植物进化出了一种受调控的细胞自杀策略，与动物的PCD不同，它是由VPE和细胞液泡介导的。
Programmed cell death (PCD) in animals depends on caspase protease activity. Plants also exhibit PCD, for example as a response to pathogens, although a plant caspase remains elusive. Here we show that vacuolar processing enzyme (VPE) is a protease essential for a virus-induced hypersensitive response that involves PCD. VPE deficiency prevented virus-induced hypersensitive cell death in tobacco plants. VPE is structurally unrelated to caspases, although VPE has a caspase-1 activity. Thus, plants have evolved a regulated cellular suicide strategy that, unlike PCD of animals, is mediated by VPE and the cellular vacuole.