In vitro regulation of circadian phosphorylation rhythm of cyanobacterial clock protein KaiC by KaiA and KaiB
In vitro regulation of circadian phosphorylation rhythm of cyanobacterial clock protein KaiC by KaiA and KaiB
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DOI：
10.1016/j.febslet.2010.01.016
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发表时间：
2010-03-05
期刊：
影响因子：
3.5
通讯作者：
Kondo, Takao
中科院分区：
文献类型：
--
作者：
Nakajima, Masato;Ito, Hiroshi;Kondo, Takao
KaiC磷酸化的生化昼夜振荡是蓝藻生物钟的中心振荡器，它通过混合三个KAI蛋白和ATP而被证明。在体内，Kaib和KaiC的细胞内水平以昼夜节律的方式振荡。通过仔细研究KAI蛋白浓度范围内的KaiC磷酸化节律，发现KaiA和Kaib分别是KaiC磷酸化节律的“参数调节”和“状态转换”调节因子。我们的结果还表明，细胞生物钟与细胞内KAI蛋白水平的昼夜变化可能存在一种夹带机制。(C)2010年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Biochemical circadian oscillation of KaiC phosphorylation, by mixing three Kai proteins and ATP, has been proven to be the central oscillator of the cyanobacterial circadian clock. In vivo, the intracellular levels of KaiB and KaiC oscillate in a circadian fashion. By scrutinizing KaiC phosphorylation rhythm in a wide range of Kai protein concentrations, KaiA and KaiB were found to be "parametertuning" and "state-switching" regulators of KaiC phosphorylation rhythm, respectively. Our results also suggest a possible entrainment mechanism of the cellular circadian clock with the circadian variation of intracellular levels of Kai proteins. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.