Expression of polydnavirus genes from the parasitoid wasp Cotesia kariyai in two noctuid hosts
Expression of polydnavirus genes from the parasitoid wasp Cotesia kariyai in two noctuid hosts
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DOI：
10.1111/j.1365-2583.1994.tb00156.x
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发表时间：
1994-01-01
影响因子：
2.6
通讯作者：
Tanaka, T.
中科院分区：
文献类型：
--
作者：
Hayakawa, Yoichi;Yazaki, K.;Tanaka, T.
从寄生蜂Cotesia kariyai分离的多DNA病毒颗粒中纯化的DNA含有分子量多分散的双链闭合环状分子。病毒注射入宿主粘虫幼虫后2天，在所有测试的幼虫组织中检测到病毒DNA，包括血细胞、达特体、神经索和脑。在注射病毒的幼虫的所有组织中也观察到病毒转录本。最具特异性的组织是血细胞，因为比其他组织中检测到更多的病毒DNA和RNA。在注射病毒的C.卡里亚病毒（CkV）基因表达发生在普通地老虎的幼虫中。目前的数据表明，在习惯性宿主粘虫的所有测试组织中均检测到。
DNA purified from polydnavirus particles isolated from the parasitoid wasp Cotesia kariyai contained double-stranded closed circular molecules which were polydisperse in molecular weight. 2 days after viral injection into the host armyworm larvae viral DNA was detected in all tested larval tissues, including haemocytes, tat body, nerve cord and brain. Viral transcripts were also observed in all the tissues of virus-injected larvae. The most specific tissue was haemocytes because more viral DNA and RNA was detected than in the other tissues. Viral transcripts were not detected in haemocytes of virus-injected that C. kariyai virus (CkV) gene expression occurs larvae of common cutworm. The present data suggest detected in all tested tissues of the habitual host armyworm.