HUMORAL IMMUNE-RESPONSE TO PLASMID PROTEIN PGP3 IN PATIENTS WITH CHLAMYDIA-TRACHOMATIS INFECTION
HUMORAL IMMUNE-RESPONSE TO PLASMID PROTEIN PGP3 IN PATIENTS WITH CHLAMYDIA-TRACHOMATIS INFECTION
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DOI：
10.1128/iai.62.12.5491-5497.1994
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发表时间：
1994-12-01
影响因子：
3.1
通讯作者：
RATTI, G
中科院分区：
文献类型：
--
作者：
COMANDUCCI, M;MANETTI, R;RATTI, G
我们通过双向电泳分析和微测序鉴定了沙眼衣原体原体的一个蛋白质，该蛋白质对应于开放阅读框3（ORF 3）编码的多肽（pgp 3）。氨基酸分析表明，在天然蛋白质中发现的第一个残基（干）是由第二个ORF 3密码子编码的残基，这意味着第一个Met残基的典型细菌去除。通过在大肠杆菌中过表达ORF 3并在非变性条件下从周质提取物中纯化产物，获得了相对大量的稳定的水溶性形式的重组pgp 3（r-pgp 3）。这些r-pgp 3制剂允许通过酶联免疫吸附试验特异性检测抗pgp 3抗体。本文分析了170份健康献血员和C.沙眼衣原体和肺炎衣原体的研究表明，大多数人对pgp 3产生免疫应答（约20%）。81%）的性传播疾病患者的沙眼衣原体血清阳性，通常与表面抗原的反应有关。r-pgp 3与C.检测到肺炎阳性血清。
We identified, by two-dimensional electrophoretic analysis and microsequencing, a protein of Chlamydia trachomatis elementary bodies which corresponds to the polypeptide (pgp3) encoded by open reading frame 3 (ORF3). Amino acid analysis showed that the first residue (dry) found in the native protein is the one encoded by the second ORF3 codon, implying a typical bacterial removal of the first Met residue. Relatively large amounts of recombinant pgp3 (r-pgp3) in a stable, water-soluble form were obtained by overexpressing ORF3 in Escherichia call and purifying the product from periplasmic extracts under nondenaturing conditions. These r-pgp3 preparations allowed specific detection of anti-pgp3 antibodies by enzyme-linked immunosorbent assay. Analysis of a group of 170 sera from healthy blood donors and from patients who were seropositive or -negative for C. trachomatis and Chlamydia pneumoniae showed that an immune response to pgp3 occurs in the majority (ca. 81%) of patients with sexually transmitted diseases who are seropositive for C, trachomatis and generally correlates with the response to tell surface antigens. No reaction between r-pgp3 and C. pneumoniae-positive sera was detected.