Monitoring DNA hybridization on alkyl modified silicon surface through capacitance measurement
Monitoring DNA hybridization on alkyl modified silicon surface through capacitance measurement
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DOI：
10.1016/s0956-5663(03)00002-2
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发表时间：
2003-08-15
影响因子：
12.6
通讯作者：
Zhao, XS
中科院分区：
文献类型：
--
作者：
Wei, F;Sun, B;Zhao, XS
单链寡脱氧核苷酸通过肽键连接至烷基修饰的硅表面。寡脱氧核苷酸修饰的硅基板用作电化学电池系统中的工作电极。用含有互补寡脱氧核苷酸链的溶液处理电极后，莫特-肖特基测量显示电极的平带电势有明显的负移，而在用非互补寡脱氧核苷酸溶液处理的对照实验中，不会发生这种偏移。 DNA 杂交也体现在实时电容测量中。基于电容测量的 DNA 传感器可能比基于荧光检测的 DNA 传感器更方便。 (C) 2003 Elsevier Science B.V. 保留所有权利。
Single strand oligodeoxynucleotide is attached to the alkyl modified silicon surface through a peptide bond. The oligodeoxynucleotide-modified silicon substrate is used as a working electrode in an electrochemical cell system. After the electrode is treated by a solution containing strands of complementary oligodeoxynucleotide the Mott-Schottky measurements exhibit obvious negative shift in the flat band potential of the electrode, while in a control experiment treated with a solution of non-complementary oligodeoxymicleotide such a shift does not occur. The DNA hybridization is also manifested in a real time capacitance measurement. A DNA sensor based on the capacitance measurement could be more convenient than that based on a fluorescence detection. (C) 2003 Elsevier Science B.V. All rights reserved.