Interplay between velocity and travel distance of kinesin-based transport in the presence of tau.
Interplay between velocity and travel distance of kinesin-based transport in the presence of tau.
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tau 存在时基于驱动蛋白的运输的速度和行进距离之间的相互作用。
DOI：
10.1016/j.bpj.2013.10.006
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发表时间：
2013
影响因子：
3.4
通讯作者：
Nemec,Brian
中科院分区：
文献类型：
--
作者：
Xu,Jing;King,StephenJ;Lapierre-Landry,Maryse;Nemec,Brian
虽然已知与疾病相关的微管相关蛋白tau在体外严重抑制基于动蛋白的转运，但逆转这一有害影响以维持细胞内健康转运的潜在机制仍不清楚。在这里，我们报告了尽管tau的存在，通过降低单动蛋白速度，多动蛋白旅行距离的明确上调。有趣的是，tau的存在也适度地降低了多动素运输中的货物速度，我们的随机模拟表明，tau介导的单动素运输的减少是这一观察结果的基础。综上所述，我们的观察结果强调了速度和运动蛋白运输的旅行距离之间的不平凡的相互作用，并表明单一运动蛋白速度是调节tau对多运动蛋白旅行距离的影响的一个有前途的实验手柄。
Although the disease-relevant microtubule-associated protein tau is known to severely inhibit kinesin-based transport in vitro, the potential mechanisms for reversing this detrimental effect to maintain healthy transport in cells remain unknown. Here we report the unambiguous upregulation of multiple-kinesin travel distance despite the presence of tau, via decreased single-kinesin velocity. Interestingly, the presence of tau also modestly reduced cargo velocity in multiple-kinesin transport, and our stochastic simulations indicate that the tau-mediated reduction in single-kinesin travel underlies this observation. Taken together, our observations highlight a nontrivial interplay between velocity and travel distance for kinesin transport, and suggest that single-kinesin velocity is a promising experimental handle for tuning the effect of tau on multiple-kinesin travel distance.