Utilization of pre-existing energy stores of female Aedes aegypti mosquitoes during the first gonotrophic cycle
Utilization of pre-existing energy stores of female Aedes aegypti mosquitoes during the first gonotrophic cycle
复制标题
DOI：
10.1016/j.ibmb.2004.05.009
复制
发表时间：
2004-09-01
影响因子：
3.8
通讯作者：
Wells, MA
中科院分区：
文献类型：
--
作者：
Zhou, GL;Pennington, JE;Wells, MA
能量储备在调节雌蚊对血粉蛋白的利用中可能起重要作用。确定的命运储备来自第一gonotrophic周期期间的糖粉和幼虫的食物将有助于阐明幼虫和成人的营养生存和繁殖的相对贡献。我们测量分配前血餐储备卵子生产或能源生产在第一gonotrophic周期使用[C-14]标记的雌性埃及伊蚊。喂食成人[3，4-C-14]-葡萄糖标记糖原和糖储存（类似于50%），脂质储存（类似于25%），蛋白质和氨基酸储存（类似于25%）。在第一个生殖营养周期中，约60%的糖原和糖储存被代谢，并全部用于能量生产。大约33%的标记蛋白质和72%的标记氨基酸储存被代谢，其中约9%被转移到鸡蛋中，其余被氧化。大约30%的脂质被代谢，大约65%被转移到鸡蛋中，其余的被氧化。饲料[1-C-14]-油酸的幼虫有效地标记成人脂质商店与约75%的标签在脂质商店和16%的蛋白质和6%的糖原。在第一个性腺营养周期中，约35%的标记脂质储存被代谢，等量被氧化并转移到卵子中。其他标记为脂肪酸的母体储存物在第一个生殖营养周期中均未被代谢。这些结果表明，碳水化合物储备是第一个性腺营养周期的关键能量来源，而脂质储备同样用于能量生产和提供鸡蛋。(C)2004爱思唯尔有限公司保留所有权利。
Pre-existing energy reserves may play an important role in regulating the utilization of blood meal proteins in female anautogenous mosquitoes. Determining the fate of reserves derived from the sugar meal and larval food during the first gonotrophic cycle would help to elucidate the relative contributions of larval and adult nutrition to survival and reproduction. We measured the allocation of pre-blood-meal reserves to egg production or energy production during the first gonotrophic cycle by using [C-14]-labeled female Aedes aegypti mosquitoes. Feeding adults [3,4-C-14]-glucose labeled the glycogen and sugar stores (similar to50%), lipid stores (similar to25%), and protein and amino acid stores (similar to25%). During the first gonotrophic cycle, about 60% of the glycogen and sugar stores were metabolized and all were used for energy production. About 33% of the labeled protein and 72% of the labeled amino acid stores were metabolized, with about 9% being transferred to the eggs and the rest oxidized. About 30% of the lipid was metabolized, with about 65% being transferred to the eggs and the rest oxidized. Feeding [1-C-14]-oleic acid to larvae effectively labeled adult lipid stores with about 75% of the label in lipid stores and 16% in proteins and 6% in glycogen. During the first gonotrophic cycle, about 35% of the labeled lipid stores were metabolized, with equal amounts being oxidized and transferred to the eggs. None of the other maternal stores labeled by fatty acid were metabolized during the first gonotrophic cycle. These results show that carbohydrate reserves are a critical source of energy during the first gonotrophic cycle, while lipid reserves are used equally for energy production and provisioning the eggs. (C) 2004 Elsevier Ltd. All rights reserved.