Evaluation of serum concentrations of biomarkers of skeletal metabolism and results of radiography as indicators of severity of osteochondrosis in foals
Evaluation of serum concentrations of biomarkers of skeletal metabolism and results of radiography as indicators of severity of osteochondrosis in foals
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DOI：
10.2460/ajvr.2004.65.143
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发表时间：
2004-02-01
影响因子：
1
通讯作者：
McIlwraith, W
中科院分区：
文献类型：
--
作者：
Billinghurst, RC;Brama, PAJ;McIlwraith, W
目的：确定骨骼代谢生物标志物的血清浓度是否可以与影像学评估相结合，表明发育中的马骨软骨病的严重程度。动物：43头荷兰温血马驹，骨软骨病的严重程度不同。24头马驹监测5个月，19头马驹监测11个月。每月的股髌股胫和胫关节x线片对骨软骨异常进行分级。对一系列血液样本进行8种软骨和骨生物标志物的检测。在监测期结束时，检查马驹的宏观骨软骨病病变。结果：某些血清生物标志物与马驹第一年骨软骨病严重程度之间存在明显的时间关系。5月龄时，胶原降解的生物标志物（胶原酶生成的ii型胶原片段的新表位，型和-II型胶原片段[COL2-3/4C（短）]和交联末端肽片段i型胶原）和骨矿化（骨钙素）是骨软骨病严重程度的阳性指标。在11月龄有病变的马驹中，骨软骨病的严重程度与COL2-3/4C（短）和骨钙素呈负相关，与ii型前胶原（CPII）的c -前肽呈正相关，CPII是胶原合成标志物。骨软病病变的影像学分级与两组年龄的宏观骨软病严重程度评分显著相关，且在5个月和11个月时联合使用骨钙素和CPII时最强。结论和临床意义：血清生物标志物指示骨软病严重程度的能力似乎取决于疾病的分期，并与x线摄影加强。在有更多永久性病变的老马驹中，骨软骨病的严重程度与骨形成减少和软骨合成增加的生物标志物浓度显著相关。
Objective-To determine whether serum concentrations of biomarkers of skeletal metabolism can, in conjunction with radiographic evaluation, indicate severity of osteochondrosis in developing horses.Animals-43 Dutch Warmblood foals with varying severity of osteochondrosis.Procedure-24 foals were monitored for 5 months and 19 foals were monitored for 11 months. Monthly radiographs of femoropatellar-femorotibial and tibiotarsal joints were graded for osteochondral abnormalities. Serial blood samples were assayed for 8 cartilage and bone biomarkers. At the end of the monitoring period, foals were examined for macroscopic osteochondrosis lesions.Results-Temporal relationships were evident between certain serum biomarkers and osteochondrosis severity in foals during their first year. Biomarkers of collagen degradation (collagenase-generated neoepitopes of type-II collagen fragments, typed and -II collagen fragments [COL2-3/4C(short)] and cross-linked telopeptide fragments type-I collagen) and bone mineralization (osteocalcin) were positive indicators of osteochondrosis severity at 5 months of age. In foals with lesions at 11 months of age, osteochondrosis severity correlated negatively with COL2-3/4C(short) and osteocalcin and positively with C-propepticle of type-II procollagen (CPII), a collagen synthesis marker. Radiographic grading of osteochondrosis lesions significantly correlated with macroscopic osteochondrosis severity score at both ages and was strongest when combined with osteocalcin at 5 months and CPII at 11 months.Conclusions and Clinical Relevance-The ability of serum biomarkers to indicate osteochondrosis severity appears to depend on stage of disease and is strengthened with radiography. In older foals with more permanent lesions, osteochondrosis severity is significantly related to biomarker concentrations of decreased bone formation and increased cartilage synthesis.