Nucleic acid and protein mass mapping by live-cell deep-ultraviolet microscopy
Nucleic acid and protein mass mapping by live-cell deep-ultraviolet microscopy
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DOI：
10.1038/nmeth1053
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发表时间：
2007-07-01
期刊：
影响因子：
48
通讯作者：
Matsudaira, Paul
中科院分区：
文献类型：
--
作者：
Zeskind, Benjamin J.;Jordan, Caroline D.;Matsudaira, Paul
我们开发了一种深紫外线（UV）显微镜，能够成像细胞有丝分裂和运动在280 nm的45分钟，最小的UV诱导的毒性，并为6小时前可见的细胞死亡在培养的人类和小鼠细胞的发病。结合将每个像素的强度转换为质量估计的计算方法，深紫外显微镜图像生成未标记细胞中核酸质量、蛋白质质量和荧光产率的地图。
We developed a deep-ultraviolet (UV) microscope capable of imaging cell mitosis and motility at 280 nm for 45 min with minimal UV-induced toxicity, and for 6 h before the onset of visible cell death in cultured human and mouse cells. Combined with computational methods that convert the intensity of each pixel into an estimate of mass, deep-UV microscopy images generate maps of nucleic acid mass, protein mass and fluorescence yield in unlabeled cells.