Metabolic activation of chrysene in mouse skin: evidence for the involvement of a triol-epoxide.
Metabolic activation of chrysene in mouse skin: evidence for the involvement of a triol-epoxide.
复制标题
小鼠皮肤中的代谢激活：三醇环氧化物参与的证据。
DOI：
10.1093/carcin/4.12.1639
复制
发表时间：
1983
期刊：
影响因子：
4.7
通讯作者：
Grover,PL
中科院分区：
文献类型：
--
作者：
Hodgson,RM;Weston,A;Grover,PL
All three possible dihydrodiols of chrysene and a chrysene triol, formed from the further metabolism of the chrysene-1,2-diol, were detected when ether extracts of mouse skin that had been treated with3-labelled chrysene were examined by h.p.l.c. The major deoxyribonucleoside-hydrocarbon adducts present in hydrolysates of DNA isolated from the mouse skin were examined by chromatography on Sephadex LH20 and by h.p.l.c. on Zorbax ODS. One adduct had chromato-graphic properties identical to those of the major adduct formed whenr-1,t-2-dihydroxy-t-3,4-oxy-1,2,3,4-tetrahydrochrysene reacts with DNA. A second major adduct was present that had chromatographic properties that were indistinguishable from those of an adduct that was formed when either chrysene-1,2-diol or 3-hydroxychrysene were incubated with DNA in a rat liver microsomal metabolising system. The results provide evidence that this new adduct is formed via the reaction of a ‘triol-epoxide’, that appears to be 9-hydroxychrysene-1,2-diol 3,4-oxide, with DNA in mouse skin.
