Time-resolved methods in biophysics. 8. Frequency domain fluorometry: applications to intrinsic protein fluorescence.
Time-resolved methods in biophysics. 8. Frequency domain fluorometry: applications to intrinsic protein fluorescence.
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生物物理学中的时间分辨方法。
DOI：
10.1039/b804450n
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Jameson,DavidM
中科院分区：
文献类型：
--
作者：
Ross,JustinA;Jameson,DavidM
时间分辨荧光光谱是化学、物理和生物科学中研究亚皮秒到微秒时间尺度的快速动力学过程不可缺少的工具。本文综述了时间分辨荧光的频域方法的发展和现代实现。将考虑强度衰减（寿命）和各向异性衰减（动态极化），并强调它们在本征蛋白荧光中的应用。特别地，我们将讨论芳香氨基酸、色氨酸、酪氨酸和苯丙氨酸的光物理学，它们负责固有的蛋白质荧光。本讨论将以几个蛋白质系统的频域研究为例加以说明。
Time-resolved fluorescence spectroscopy is an indispensable tool in the chemical, physical and biological sciences for the study of fast kinetic processes in the subpicosecond to microsecond time scale. This review focuses on the development and modern implementation of the frequency domain approach to time-resolved fluorescence. Both intensity decay (lifetime) and anisotropy decay (dynamic polarization) will be considered and their application to intrinsic protein fluorescence will be highlighted. In particular we shall discuss the photophysics of the aromatic amino acids, tryptophan, tyrosine and phenylalanine, which are responsible for intrinsic protein fluorescence. This discussion will be illustrated with examples of frequency domain studies on several protein systems.