Structural analysis of the RSC chromatin-remodeling complex
Structural analysis of the RSC chromatin-remodeling complex
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DOI：
10.1073/pnas.162504299
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发表时间：
2002-10-15
影响因子：
11.1
通讯作者：
Lorch, Y
中科院分区：
文献类型：
--
作者：
Asturias, FJ;Chung, WH;Lorch, Y
RSC染色质重塑复合体的电子显微镜显示，在中心空腔周围有一个蛋白质密度环。空腔的大小和形状与核小体非常相似。核酸酶保护分析结果与腔内核小体结合一致。这种结合可以解释RSC在ATP存在下暴露核小体DNA而不丢失相关组蛋白的能力。
Electron microscopy of the RSC chromatin-remodeling complex reveals a ring of protein densities around a central cavity. The size and shape of the cavity correspond closely to those of a nucleosome. Results of nuclease protection analysis are consistent with nucleosome binding in the cavity. Such binding could explain the ability of RSC to expose nucleosomal DNA in the presence of ATP without loss of associated histones.