Natural variation of ZmHKT1 affects root morphology in maize at the seedling stage
Natural variation of ZmHKT1 affects root morphology in maize at the seedling stage
复制标题
ZmHKT1 的自然变异影响玉米幼苗期的根系形态
DOI：
10.1007/s00425-018-3043-2
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发表时间：
2019-03-01
期刊：
影响因子：
4.3
通讯作者：
Yang, Zefeng
中科院分区：
文献类型：
--
作者：
Li, Pengcheng;Pan, Ting;Yang, Zefeng
ZmHKT 1基因启动子区的8个变异体与根直径显著相关，基于这些变异体共发现4种单倍型，其中Hap 2的根直径最大。根系在植物吸收水分、养分和固着等方面起着重要作用。鉴定与根系性状相关的基因和相应的SNPs将能够开发具有更好根系性状的玉米品系，这可能有助于提高吸收养分和水分的能力。对349份玉米自交系的耐盐基因ZmHKT 1进行了全基因组测序，并分析了其核苷酸多态性与苗期根系性状的相关性。ZmHKT 1共检测到269个变异体，其中单核苷酸多态性226个，插入和缺失43个。该基因具有较高的核苷酸多样性，尤其是在非基因区。ZmHKT 1非翻译区共有19个变异与6个幼苗性状相关。启动子区有8个变异体与平均根直径（ARD）显著相关，基于这些变异体共发现4种单倍型，其中Hap 2的ARD最大。在高连锁不平衡（SNP-415和SNP 2169）与多效性效应的SNP显着相关株高，根表面积，根体积，地上部干重。这一结果表明，ZmHKT 1是玉米苗期根系性状表型变异的重要贡献者，这些显著变异可用于开发改良根系性状的功能标记。
Eight variants in ZmHKT1 promoter were significantly associated with root diameter, four haplotypes based on these significant variants were found, and Hap2 has the largest root diameter. Roots play an important role in uptake of water, nutrients and plant anchorage. Identification of gene and corresponding SNPs associated with root traits would enable develop maize lines with better root traits that might help to improve capacity for absorbing nutrients and water acquisition. The genomic sequences of a salt tolerance gene ZmHKT1 was resequenced in 349 maize inbred lines, and the association between nucleotide polymorphisms and seedling root traits was detected. A total of 269 variants in ZmHKT1 were identified, including 226 single nucleotide polymorphisms and 43 insertions and deletions. The gene displayed high level of nucleotide diversity, especially in non-genic regions. A total of 19 variations in untranslated region of ZmHKT1 were found to be associated with six seedling traits. Eight variants in promoter region were significantly associated with average root diameter (ARD), four haplotypes were found based on these significant variants, and Hap2 has the largest ARD. Two SNPs in high-linkage disequilibrium (SNP-415 and SNP 2169) with pleiotropic effects were significantly associated with plant height, root surface area, root volume, and shoot dry weight. This result revealed that ZmHKT1 was an important contributor to the phenotypic variations of seedling root traits in maize, these significant variants could use to develop functional markers to improve root traits.