Pathways of transport protein evolution: recent advances.
Pathways of transport protein evolution: recent advances.
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运输蛋白演变的途径：最近的进步。
DOI：
10.1515/bc.2011.018
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发表时间：
2011-01
影响因子：
3.7
通讯作者：
Saier MH Jr
中科院分区：
文献类型：
--
作者：
Lam VH;Lee JH;Silverio A;Chan H;Gomolplitinant KM;Povolotsky TL;Orlova E;Sun EI;Welliver CH;Saier MH Jr
我们在此报告我们对转运蛋白进化的理解的最新进展。药物代谢物转运蛋白 (DMT) 超家族 (TC# 2.A.7) 源于 2TMS 前体，产生 4TMS 蛋白，然后添加 1 个并复制得到 10 个。建议的途径是 2 –> 4 –> 5 –> 10。这个超家族提供了一个罕见的例子，其中该进化途径中所有建议的拓扑中间体均已在当前的蛋白质数据库中得到鉴定。另一个家族，寡肽转运蛋白 (OPT) 家族 (TC# 2.A.67)，也以 2 个 TMS 肽前体开始，但它遵循以下途径：在当前数据库中仅识别出 16 和 17 个 TMS OPT 家族成员。 K+ 通道的 TRIC 家族以动物为特征，通过以下途径产生：其中第七个 TMS 在 C 端添加到由 3 个 TMS 重复产生的 6 个 TMS 前体上。令人惊讶的是，动物 TRIC 通道被证明在原核生物中具有大量 7 TMS 同源物，而这些同源物以前从未被鉴定过。我们发现两个完整膜蛋白家族产生了多种当前的拓扑类型。 SdpC 杀伤因子免疫蛋白家族的成员 SdpI (TC# 9.A.32) 可能通过以下途径产生：而血红素处理蛋白 (HHP) 家族 (TC# 9.B.14) 的成员通过以下途径产生： 还预测了迄今为止在 P-ATP 酶超家族中鉴定出的七种拓扑类型的 P 型 ATP 酶的进化途径。最后，6TMS 普遍存在的 CDF 载体（TC# 1.A.4）可能通过前两个 TMS 的丢失而产生了 4TMS 的 CRAC 通道，这是逆向进化的一个不寻常的例子。
We herein report recent advances in our understanding of transport protein evolution. The Drug-Metabolite Transporter (DMT) superfamily (TC# 2.A.7) arose from a 2TMS precursor to give 4TMS proteins which then added one and duplicated to give 10. The proposed pathway is 2 –> 4 –> 5 –> 10. This superfamily provides a rare example where all proposed topological intermediates in this evolutionary pathway have been identified in current protein databases. Another family, the Oligopeptide Transporter (OPT) family (TC# 2.A.67), also started with a 2 TMS peptide precursor, but it followed the pathway: Only 16 and 17 TMS OPT family members have been identified in current databases. The TRIC family of K+ channels, characterized in animals, arose via the pathway: where the seventh TMS was added c-terminally to the 6 TMS precursor that resulted from a 3 TMS duplication. Surprisingly, animal TRIC channels proved to have numerous 7 TMS homologues in prokaryotes, none of which had been identified previously. We found that two families of integral membrane proteins gave rise to multiple current topological types. Members of the SdpC killer factor immunity protein family, SdpI (TC# 9.A.32) probably arose via the pathway: while members of the Heme Handling Protein (HHP) Family (TC# 9.B.14) arose via the pathway: Predictions are also made for an evolutionary pathway giving rise to the seven topological types of P-type ATPases so far identified in the P-ATPase superfamily. Finally, the ubiquitous CDF carriers (TC# 1.A.4) of 6TMSs probably gave rise to CRAC channels of 4TMSs by loss of the first two TMSs an unusual example of retroevolution.