Behavior of male-specific minor histocompatibility antigen in skin and limb transplantation
Behavior of male-specific minor histocompatibility antigen in skin and limb transplantation
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DOI：
10.1016/s0022-4804(03)00136-7
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发表时间：
2003-11-01
影响因子：
2.2
通讯作者：
Kawai, S
中科院分区：
文献类型：
--
作者：
Muramatsu, K;Kurokawa, Y;Kawai, S
背景虽然男性特异性次要组织相容性抗原H-Y在实验和临床器官移植中的作用已被越来越多的人所了解，但对肌肉骨骼组织移植的研究却很少。本研究旨在描述雄性特异性次要组织相容性H-Y抗原在大鼠皮肤和全肢移植中的行为。使用三种不同品系的近交系大鼠（刘易斯、F344和Dark Agglutinus），将75个供体后肢和18个皮肤移植物同基因移植到性别不匹配的受体。术后观察受体长达48周。通过移植肢体皮肤外观和组织学监测排斥反应。用Y染色体特异性引物进行聚合酶链反应检测微嵌合体。所有肢体移植受者均未发生皮肤排斥反应，48周内，组织学检查未显示肢体移植物的所有成分发生任何排斥反应。预期功能恢复成功。在未处理的雌性受体的淋巴组织中检测到稳定和高水平的嵌合体（>1%）。刘易斯和F344女性受体在术后6周内对男性皮肤移植物产生排斥反应。所有女性皮肤移植在男性患者中均存活。结果提示，H-Y抗原在大鼠皮肤移植中可引起排斥反应，而在全肢移植中不引起排斥反应。系统嵌合体可能在性别不匹配的肢体移植物的接受中起重要作用。(C)2003年爱思唯尔公司All rights reserved.
Background. Although the role of male-specific minor histocompatibility antigen H-Y has been increasingly understood in both experimental and clinical organ transplantation, little has been investigated on musculoskeletal tissue transplantation. This study was performed to describe the behavior of male-specific minor histocompatibility H-Y antigen in rat skin and whole limb transplantation.Materials and methods. Using three different strains of inbred rats (Lewis, F344, and Dark Agouti), 75 donor hindlimbs and eighteen skin grafts were isogenically transplanted to the sex-mismatched recipients. Recipients were observed up to 48 weeks postoperatively. Rejection was monitored by the appearance of the skin of the grafted limb and histology. Systemic microchimerism was assessed by polymerase chain reaction using Y-chromosome specific primers.Results. Skin rejection didn't occur in all limb transplant recipients and histology did not show any rejection findings in all components of the limb graft through 48 weeks. Successful functional recovery was expected. Stable and high level of chimerism (>1%) was detected in the lymphoid tissues in nontreated female recipients. Male skin grafts were rejected by Lewis and F344 female recipients within 6 weeks postoperatively. All female skin grafts survived in male recipients.Conclusion. Our results suggest that H-Y antigen can induce graft rejection in rat skin graft but causes no rejection reaction in whole limb transplantation. Systemic chimerism may play an important role for acceptance of sex-mismatched limb graft. (C) 2003 Elsevier Inc. All rights reserved.