Osteoarthritic changes in the biphasic mechanical properties of the chondrocyte pericellular matrix in articular cartilage
Osteoarthritic changes in the biphasic mechanical properties of the chondrocyte pericellular matrix in articular cartilage
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DOI：
10.1016/j.jbiomech.2004.04.012
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发表时间：
2005-03-01
影响因子：
2.4
通讯作者：
Guilak, F
中科院分区：
文献类型：
--
作者：
Alexopoulos, LG;Williams, GM;Guilak, F
细胞周基质（PCM）是关节软骨中包围软骨细胞的狭窄软骨组织区域。先前的建模研究表明，PCM相对于细胞外基质（ECM）的机械性能可以显着影响软骨细胞的应力-应变，流体流动和物理化学环境，这表明PCM在关节软骨中起着生物力学作用。本研究的目的是测量PCM的机械性能，使用微量吸液管抽吸加上线性双相有限元模型，并确定骨关节炎（OA）的PCM的机械性能的改变。使用最近开发的分离技术，软骨（软骨细胞及其PCM）从非退化和骨关节炎的人软骨中机械提取。PCM的瞬态力学行为由双相模型很好地描述，表明PCM的粘弹性响应可归因于与ECM类似的流动依赖性效应。随着OA，PCM的平均杨氏模量显著降低（38.7 +/- 16.2 kPa vs. 23.5 +/- 12.9 kPa，p < 0.001），并且渗透性显著升高（4.19 +/- 3.78 x 10（-17）m（4）/N s对比10.2+/-9.38 x 10（-11）m（4）/N s，p < 0.001）。非退化和OA PCM的泊松比相似（0.044 ± 0.063 vs. 0.030 ± 0.068，p > 0.6）。这些结果表明，PCM可能会经历退行性过程与OA，类似于那些发生在ECM。结合以前的理论模型的细胞-基质相互作用在软骨中，我们的研究结果表明，在PCM与OA的属性的变化可能有一个重要的影响软骨细胞的生物力学环境。(C)2004爱思唯尔有限公司保留所有权利。
The pericellular matrix (PCM) is a narrow region of cartilaginous tissue that surrounds chondrocytes in articular cartilage. Previous modeling studies indicate that the mechanical properties of the PCM relative to those of the extracellular matrix (ECM) can significantly affect the stress-strain, fluid flow, and physicochemical environments of the chondrocyte, suggesting that the PCM plays a biomechanical role in articular cartilage. The goals of this study were to measure the mechanical properties of the PCM using micropipette aspiration coupled with a linear biphasic finite element model, and to determine the alterations in the mechanical properties of the PCM with osteoarthritis (OA). Using a recently developed isolation technique, chondrons (the chondrocyte and its PCM) were mechanically extracted from non-degenerate and osteoarthritic human cartilage. The transient mechanical behavior of the PCM was well-described by a biphasic model, suggesting that the viscoelastic response of the PCM is attributable to flow-dependent effects, similar to that of the ECM. With OA, the mean Young's modulus of the PCM was significantly decreased (38.7 +/- 16.2 kPa vs. 23.5 +/- 12.9 kPa, p < 0.001), and the permeability was significantly elevated (4.19 +/- 3.78 x 10(-17)m(4)/N s vs. 10.2+/-9.38 x 10(-11)m(4) /N s, p < 0.001). The Poisson's ratio was similar for both non-degenerate and OA PCM (0.044 + 0.063 vs. 0.030 +/- 0.068, p > 0.6). These findings suggest that the PCM may undergo degenerative processes with OA, similar to those occurring in the ECM. In combination with previous theoretical models of cell-matrix interactions in cartilage, our findings suggest that changes in the properties of the PCM with OA may have an important influence on the biomechanical environment of the chondrocyte. (C) 2004 Elsevier Ltd. All rights reserved.