Evaluation of phytotoxicity and ecotoxicity potentials of a cyanobacterial extract containing microcystins under realistic environmental concentrations and in a soil-plant system
Evaluation of phytotoxicity and ecotoxicity potentials of a cyanobacterial extract containing microcystins under realistic environmental concentrations and in a soil-plant system
复制标题
DOI：
10.1016/j.chemosphere.2015.02.008
复制
发表时间：
2015-06-01
期刊：
影响因子：
8.8
通讯作者：
Bouaiecha, Noureddine
中科院分区：
文献类型：
--
作者：
Corbel, Sylvain;Mougin, Christian;Bouaiecha, Noureddine
研究了含有14种微囊藻毒素变异体的铜绿微囊藻（Microcystis aeruginosa，PCC 7820）粗提物对番茄（Solanum lycopersicum，MicroTom和Saint-Pierre）、小麦（Triticum aestivum）和莴苣（Lactuca sativa）种子萌发和胚根发育的影响。此外，14天暴露于含有实际浓度的微囊藻毒素（0-0.1 mg eq.微囊藻毒素-LRL-1）对番茄MicroTom幼苗生长的影响进一步在根和地上部分生物量上进行评估。研究了土壤微生物参数，如胞外酶活性、硝化活性和氨氧化微生物丰度的变化。在发芽试验中，蓝藻提取物仅抑制小麦种子的发芽，其EC 50为11 mg eq.微囊藻毒素-LR L-1，比氯化镉低13倍（EC 50为145 mg L-1）。此外，含有低浓度的微囊藻毒素的蓝藻提取物增加了初生根的生长，然而，高浓度的降低，除了小麦的所有植物。在土壤-植物处理中，只有番茄地上部分的生物量显著提高。此外，只有土壤硝化潜力和氨氧化细菌丰度受到持续影响。硝化势的增加与氨氧化细菌的丰度呈显著正相关（r = 0.56）。这项工作表明，暴露于含有真实环境微囊藻毒素浓度的蓝藻提取物可能会影响种子萌发，这取决于植物物种。首次强调了土壤细菌功能的干扰，土壤硝化过程的证据。(C)2015爱思唯尔有限公司版权所有。
The impact of a crude extract of Microcystis aeruginosa (PCC7820) containing 14 microcystin variants was investigated on seeds germination and radicles development of four agricultural plants: two tomato varieties Solanum lycopersicum (MicroTom and Saint-Pierre), the wheat Triticum aestivum and the lettuce Lactuca sativa. In addition, the effect of 14 d-exposure to irrigation water containing realistic concentrations of microcystins (0-0.1 mg eq. microcystin-LRL-1) on the tomato MicroTom seedling growth was further evaluated on roots and aerial part biomasses. Impacts on soil bacterial parameters, as such extracellular enzymatic activities, nitrification activity and abundances of ammonia-oxidizing microorganisms were also investigated. In germination-test, the cyanobacterial extract inhibited only the germination of the wheat seeds, with an EC50 of 11 mg eq. microcystin-LR L-1; which is 13 times lower than that of the cadmium chloride (EC50 of 145 mg L-1). Moreover, the cyanobacterial extract containing low concentrations of microcystins increased the growth of primary roots; however, high concentrations decreased it for all plants except for the wheat. In the soil-plant approach, only aerial part biomass of the tomato MicroTom was enhanced significantly. In addition, only soil nitrification potential and ammonia-oxidizing bacterial abundances were consistently impacted. A significant positive correlation (r = 0.56) was found between the increase of nitrification potential and abundances of ammonia-oxidizing bacteria. This work suggested, that exposure to a cyanobacterial extract containing realistic environmental microcystins concentrations could affect seed germination, depending plant species. It was also highlighted, for the first time, disturbances in soil bacteria functioning, evidences on soil nitrification process. (C) 2015 Elsevier Ltd. All rights reserved.