Nitrogen-to-protein conversion factors revisited for applications of microalgal biomass conversion to food, feed and fuel
Nitrogen-to-protein conversion factors revisited for applications of microalgal biomass conversion to food, feed and fuel
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DOI：
10.1016/j.algal.2015.07.013
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发表时间：
2015-09-01
影响因子：
5.1
通讯作者：
Laurens, Lieve M. L.
中科院分区：
文献类型：
--
作者：
Templeton, David W.;Laurens, Lieve M. L.
准确测定蛋白质含量对于食品、饲料和燃料市场中藻类生物质的价值评估非常重要。将元素氮转化为蛋白质是一种广为接受和广泛实践的方法，但取决于开发适用的氮-蛋白质转化因子。确定该因子的最完整方法是对受试材料进行六种不同的水解，这些方法并不总是在报告的文献研究中进行。我们报告了从21个藻类样品沿着与超过50个氨基酸配置文件，代表新鲜和海洋藻类不同的培养条件下确定的保守转换因子的新数据。我们发现，不同的藻类样品之间的氨基酸谱是一致的，但菌株之间的非蛋白氮（微藻中高达54%）的大的变异性导致计算的转换因子的变异性。我们包括我们计算的新的氮蛋白质转换因子模型和商业相关的生物燃料藻株，并与文献进行比较。(C)2015年由Elsevier B. V.出版
Accurately determining protein content is important in the valorization of algal biomass in food, feed and fuel markets. Conversion of elemental nitrogen to protein is a well-accepted and widely practiced method, but depends on developing an applicable nitrogen-to-protein conversion factor. The most complete method to determine this factor takes six different hydrolyses of the subject material and these are not always carried out in reported literature studies. We report new data for conservative conversion factors determined from 21 algae samples along with over 50 amino acid profiles from the literature, representing distinct cultivation conditions for fresh and marine algae. We find that the amino acid profile among different algae samples is consistent, however the large variability between strains in non-protein nitrogen (up to 54% in microalgae) causes variability in the calculated conversion factor. We include our calculated novel nitrogen-to-protein conversion factors for model and commercially relevant biofuel algal strains and compare these with the literature. (C) 2015 Published by Elsevier B.V.