Enhanced expression of the type II transforming growth factor beta receptor in human pancreatic cancer cells without alteration of type III receptor expression.
Enhanced expression of the type II transforming growth factor beta receptor in human pancreatic cancer cells without alteration of type III receptor expression.
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发表时间：
1993-06
期刊：
影响因子：
11.2
通讯作者：
H. Friess;Y. Yamanaka;M. Büchler;H. Beger;M. Kobrin;R. L. Baldwin;M. Korc
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文献类型：
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作者：
H. Friess;Y. Yamanaka;M. Büchler;H. Beger;M. Kobrin;R. L. Baldwin;M. Korc
我们最近发现，人类胰腺癌表现出强烈的免疫染色的三种哺乳动物转化生长因子β（TGF-β）亚型。这些重要的生长调节多肽与许多蛋白质结合，包括I型TGF-β受体（T β R-I）、II型TGF-β受体（T β R-II）和III型TGF-β受体（T β R-III）。在本研究中，我们试图确定胰腺癌中T β R-II和T β R-III的表达是否改变。北方印迹分析表明，与正常胰腺相比，胰腺癌中编码T β R-II的mRNA水平增加了4.6倍（P < 0.01）。相反，编码T β R-III的mRNA水平没有增加。原位杂交显示，T β R-II mRNA在大多数癌细胞中表达，而编码T β R-III的mRNA颗粒仅在少数癌细胞中可检测到，并且主要存在于周围基质中。这些发现表明，T β R-II水平的提高可能在调节人胰腺癌细胞生长中起作用，而T β R-III可能在细胞外基质中起作用。
We have recently found that human pancreatic adenocarcinomas exhibit strong immunostaining for the three mammalian transforming growth factor beta (TGF-beta) isoforms. These important growth-regulating polypeptides bind to a number of proteins, including the type I TGF-beta receptor (T beta R-I), type II TGF-beta receptor (T beta R-II), and the type III TGF-beta receptor (T beta R-III). In the present study we sought to determine whether T beta R-II and T beta R-III expression is altered in pancreatic cancer. Northern blot analysis indicated that, by comparison with the normal pancreas, pancreatic adenocarcinomas exhibited a 4.6-fold increase (P < 0.01) in mRNA levels encoding T beta R-II. In contrast, mRNA levels encoding T beta R-III were not increased. In situ hybridization showed that T beta R-II mRNA was expressed in the majority of cancer cells, whereas mRNA grains encoding T beta R-III were detectable in only a few cancer cells and were present mainly in the surrounding stroma. These findings suggest that enhanced levels of T beta R-II may have a role in regulating human pancreatic cancer cell growth, while T beta R-III may function in the extracellular matrix.