Abscisic acid determines basal susceptibility of tomato to Botrytis cinerea and suppresses salicylic acid-dependent signaling mechanisms
Abscisic acid determines basal susceptibility of tomato to Botrytis cinerea and suppresses salicylic acid-dependent signaling mechanisms
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DOI：
10.1104/pp.010605
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发表时间：
2002-02-01
期刊：
影响因子：
7.4
通讯作者：
Höfte, MM
中科院分区：
文献类型：
--
作者：
Audenaert, K;De Meyer, GB;Höfte, MM
脱落酸（阿坝）是植物与病原菌相互作用的激素之一。在这项工作中，我们表明，番茄（Lycopersicon esculentum米尔。cv Moneymaker）突变体（sitiens植物）比野生型（WT）植物对坏死营养型真菌灰葡萄孢的抗性高得多。外源阿坝的施用恢复了对B的敏感性。灰霉病在sitiens植物和增加的敏感性WT植物。这些结果表明阿坝在番茄对B的敏感性中起主要作用。灰绿色阿坝似乎与植物对B的功能性防御反应相互作用。灰叶用转基因NahG番茄植物和苯并（1，2，3）噻二唑-7-硫代甲酸的实验证明了水杨酸在番茄-B中的重要性。灰叶相互作用此外，在感染B. cinerea，sitiens植物显示苯丙氨酸解氨酶活性的明显增加，这在感染的WT植物中没有观察到，表明健康WT番茄植物中的阿坝水平部分抑制苯丙氨酸解氨酶活性。此外，sitiens植物变得更敏感苯并（1，2，3）噻二唑-7-硫代羧酸根处理。PR 1a基因表达的阈值与野生型植物相比，在拟南芥中下降了10至100倍。因此，阿坝似乎负调节水杨酸依赖的防御途径在番茄，这可能是阿坝水平决定的敏感性B的机制之一。灰绿色
Abscisic acid (ABA) is one of the plant hormones involved in the interaction between plants and pathogens. In this work, we show that tomato (Lycopersicon esculentum Mill. cv Moneymaker) mutants with reduced ABA levels (sitiens plants) are much more resistant to the necrotrophic fungus Botrytis cinerea than wild-type (WT) plants. Exogenous application of ABA restored susceptibility to B. cinerea in sitiens plants and increased susceptibility in WT plants. These results indicate that ABA plays a major role in the susceptibility of tomato to B. cinerea. ABA appeared to interact with a functional plant defense response against B. cinerea. Experiments with transgenic NahG tomato plants and benzo(1,2,3)thiadiazole-7-carbothioic acid demonstrated the importance of salicylic acid in the tomato-B. cinerea interaction. In addition, upon infection with B. cinerea, sitiens plants showed a clear increase in phenylalanine ammonia lyase activity, which was not observed in infected WT plants, indicating that the ABA levels in healthy WT tomato plants partly repress phenylalanine ammonia lyase activity. In addition, sitiens plants became more sensitive to benzo(1,2,3)thiadiazole-7-carbothioic acid root treatment. The threshold values for PR1a gene expression declined with a factor 10 to 100 in shims compared with WT plants. Thus, ABA appears to negatively modulate the salicylic acid-dependent defense pathway in tomato, which may be one of the mechanisms by which ABA levels determine susceptibility to B. cinerea.