Effects of polyphyllin I on growth inhibition of human non-small lung cancer cells and in xenograft
Effects of polyphyllin I on growth inhibition of human non-small lung cancer cells and in xenograft
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DOI：
10.1093/abbs/gmq091
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发表时间：
2010-11-01
影响因子：
3.7
通讯作者：
Xu, Rongzhen
中科院分区：
文献类型：
--
作者：
Kong, Minjian;Fan, Junqiang;Xu, Rongzhen
从巴黎中提取的小分子单体多叶灵I（Polyphyllin I，PPI）具有较强的抗肿瘤作用。培养人肺腺癌A549细胞、人肺鳞状细胞癌SK-MES-1细胞和人肺大细胞癌H460细胞，然后用PPI处理。采用3-[4，5-二甲基噻唑-2-基]-2，5-二苯基四氮唑溴化物法、流式细胞术、western blot分析和DNA ladder检测细胞增殖和凋亡。给荷瘤裸鼠注射PPI，记录肿瘤生长情况。我们的研究结果表明，PPI显着抑制三个非小细胞肺癌（NSCLC）细胞系的增殖，与抑制浓度（IC 50）为1.24，2.40和2.33 μ g/ml的A549，H460，SK-MES-1细胞，分别。2.5 μ g/ml PPI作用24 h后，A549细胞凋亡率为39.68%，明显高于对照组。与顺铂（DDP）或PBS治疗组相比，PPI治疗组在裸小鼠中的肿瘤生长受到显着抑制。PPI在体内外均具有抗肿瘤作用，其机制可能与诱导细胞凋亡有关。
Polyphyllin I (PPI), a small molecular monomer extracted from Rhizoma of Paris polyphyllin, shows strong anticancer effects in previous study. Human lung adenocarcinoma A549 cells, human lung squamous cell carcinoma SK-MES-1 cells, and human lung large cell carcinoma H460 cells were cultured and then treated with PPI. Cell proliferation and apoptosis were measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide assay, flow cytometry, western blot analysis, and DNA ladder. Athymic nude mice bearing tumors were injected with PPI, and tumor growth was recorded. Our results showed that PPI significantly inhibited the proliferation of three non-small cell lung cancer (NSCLC) cell lines, with the inhibitory concentrations (IC50) of 1.24, 2.40, and 2.33 mu g/ml for A549, H460, and SK-MES-1 cells, respectively. After being treated with 2.5 mu g/ml of PPI for 24 h, the apoptotic rate of A549 cells was 39.68%, which was remarkably higher than that of the control. Tumor growth was significantly inhibited in the PPI-treated group compared with the group treated with cisplatin (DDP) or PBS in the nude mice. PPI exhibits antitumor ability in NSCLC cells in vitro and in vivo, which might be related to the apoptosis induced by PPI.