The effects of infusions of CART 55-102 into the basolateral amygdala on amphetamine-induced conditioned place preference in rats.
The effects of infusions of CART 55-102 into the basolateral amygdala on amphetamine-induced conditioned place preference in rats.
复制标题
将 CART 55-102 输注到基底外侧杏仁核对安非他明诱导的大鼠条件性位置偏好的影响。
DOI：
10.1007/s00213-009-1748-4
复制
发表时间：
2010
影响因子：
3.4
通讯作者：
Figge,DavidA
中科院分区：
文献类型：
--
作者：
Rademacher,DavidJ;Sullivan,ElyseM;Figge,DavidA
基本原理 d-苯丙胺 (AMPH) 的情感方面可能部分由基底外侧杏仁核 (BLA) 中的可卡因和苯丙胺调节转录物 (CART) 肽介导。环境-药物关联的形成会产生条件性位置偏爱 (CPP) 或条件性位置厌恶 (CPA)。 目的本研究的目的是确定 BLA 内输注 CART 55-102 是有益的还是厌恶的，并调节 AMPH 奖励。 材料和方法将大鼠的双侧插管植入 BLA，进行位置调节，并测试 CPP 或 CPA。大鼠接受 BLA 内输注人工脑脊髓液或三种剂量的 CART 55–102（1、2 或 4 µg/侧）之一、BLA 内输注低奖励剂量的 CART 55–102（1 µg/侧）加上注射低奖励剂量的 AMPH（0.1 mg/kg，腹腔注射）或 BLA 内输注厌恶剂量的 CART 55–102（4 µg/侧）加上奖励剂量的 AMPH（1.0 mg/kg，腹膜内注射）。结果 BLA 内输注 2 µg/侧 CART 55–102 产生 CPP，4 µg/侧产生 CPA，1 µg/侧既不产生 CPP 也不产生 CPA。 BLA 内输注低奖励剂量的 CART 55–102（1 µg/侧）加上注射低奖励剂量的 AMPH（0.1 mg/kg，腹腔注射）可产生 CPP。 BLA 内输注厌恶剂量的 CART 55–102（4 µg/侧）加上奖励剂量的 AMPH（1.0 mg/kg，腹膜内注射）既不会产生 CPP，也不会产生 CPA。结论 BLA 内 CART 55–102 的情感特性及其促进或阻止 AMPH 奖励的能力都是剂量依赖性的。
RationaleThe affective aspects ofd-amphetamine (AMPH) may be mediated, in part, by cocaine- and amphetamine-regulated transcript (CART) peptides in the basolateral amygdala (BLA). The formation of context-drug associations produces either conditioned place preference (CPP) or conditioned place aversion (CPA).ObjectivesThe aim of the present study was to determine whether intra-BLA infusions of CART 55–102 are either rewarding or aversive and modulate AMPH reward.Materials and methodsRats were implanted with bilateral cannulae in the BLA, were subjected to place conditioning, and were tested for CPP or CPA. Rats were conditioned with either intra-BLA infusions of artificial cerebral spinal fluid or one of three dose of CART 55–102 (1, 2, or 4 μg/side), intra-BLA infusions of a subrewarding dose of CART 55–102 (1 μg/side) plus injections of a subrewarding dose of AMPH (0.1 mg/kg, i.p.), or intra-BLA infusions of an aversive dose of CART 55–102 (4 μg/side) plus injections of a rewarding dose of AMPH (1.0 mg/kg, i.p.).ResultsIntra-BLA infusions of 2 μg/side CART 55–102 produced CPP, 4 μg/side produced CPA, and 1 μg/side produced neither CPP nor CPA. Intra-BLA infusions of a subrewarding dose of CART 55–102 (1 μg/side) plus injections of a subrewarding dose of AMPH (0.1 mg/kg, i.p.) produced CPP. Intra-BLA infusions of an aversive dose of CART 55–102 (4 μg/side) plus injections of a rewarding dose of AMPH (1.0 mg/kg, i.p.) produced neither CPP nor CPA.ConclusionsBoth the affective properties of intra-BLA CART 55–102 and its ability to either facilitate or block AMPH reward are dose dependent.