Intron definition in splicing of small Drosophila introns.
Intron definition in splicing of small Drosophila introns.
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小果蝇内含子剪接中的内含子定义。
DOI：
10.1128/mcb.14.5.3434-3445.1994
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发表时间：
1994
影响因子：
5.3
通讯作者：
Berget,SM
中科院分区：
文献类型：
--
作者：
Talerico,M;Berget,SM
黑腹果蝇中大约一半的内含子太小而无法在脊椎动物中发挥作用，并且通常缺乏与脊椎动物 3' 剪接位点相关的嘧啶束。在这里，我们报告了两个这样的内含子的剪接和剪接体组装特性：一个具有缺乏嘧啶的 3' 剪接位点，另一个具有富含嘧啶的 3' 剪接位点。缺乏嘧啶的内含子绝对依赖于其小尺寸来进行体内和体外剪接和组装。因此，它的特性让人想起酵母内含子。富含嘧啶的内含子具有介于酵母和脊椎动物之间的特性。当作为内含子或外显子存在时，该 3' 剪接位点定向组装 ATP 依赖性复合物，并支持中等长度内含子的低水平体内剪接。我们建议剪接位点可以被识别为跨外显子或内含子的对，具体取决于哪个距离较短，并且 3' 剪接位点上游富含嘧啶的区域有利于外显子模式。
Approximately half of the introns inDrosophila melanogasterare too small to function in a vertebrate and often lack the pyrimidine tract associated with vertebrate 3′ splice sites. Here, we report the splicing and spliceosome assembly properties of two such introns: one with a pyrimidine-poor 3′ splice site and one with a pyrimidine-rich 3′ splice site. The pyrimidine-poor intron was absolutely dependent on its small size for in vivo and in vitro splicing and assembly. As such, it had properties reminiscent of those of yeast introns. The pyrimidine-rich intron had properties intermediate between those of yeasts and vertebrates. This 3′ splice site directed assembly of ATP-dependent complexes when present as either an intron or exon and supported low levels of in vivo splicing of a moderate-length intron. We propose that splice sites can be recognized as pairs across either exons or introns, depending on which distance is shorter, and that a pyrimidine-rich region upstream of the 3′ splice site facilitates the exon mode.