The Sialic Acid Binding Protein, Hsa, in Streptococcus gordonii DL1 also Mediates Intergeneric Coaggregation with Veillonella Species.
The Sialic Acid Binding Protein, Hsa, in Streptococcus gordonii DL1 also Mediates Intergeneric Coaggregation with Veillonella Species.
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DOI：
10.1371/journal.pone.0143898
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Qi F
中科院分区：
文献类型：
--
作者：
Zhou P;Liu J;Li X;Takahashi Y;Qi F
牙齿生物膜的发展涉及最初的殖民者的牙齿表面的先锋殖民，其次是先锋和后来的殖民者之间的细胞-细胞共聚集。戈登链球菌是最早的定居者之一。除了在口腔生物膜形成中的作用外，S。戈登氏菌也是易感人群感染性心内膜炎的病原体。表面粘附素Hsa在沙门氏菌的定殖过程中起重要作用。戈登氏菌对心脏组织的作用;然而，其在口腔生物膜发育中的作用尚未报道。在这项研究中，我们证明了Hsa是必不可少的S。gordonii和韦荣球菌属，它们是连接早期殖民者和晚期殖民者的桥梁物种。有趣的是，所需的HSA结合唾液酸在人类细胞表面上的相同的结构域也需要与韦荣氏球菌的共聚集。然而，唾液酸似乎不需要这种属间的共聚集。这一结果表明，虽然Hsa的相同结构域参与结合真核细胞以及韦荣氏球菌细胞，但结合机制是不同的。同时研究了人血清白蛋白的基因表达模式，结果表明，人血清白蛋白与韦荣氏球菌共聚集并不诱导人血清白蛋白的基因表达。
Dental biofilm development involves initial colonization of the tooth’s surface by pioneer colonizers, followed by cell-cell coaggregation between the pioneer and later colonizers. Streptococcus gordonii is one of the pioneer colonizers. In addition to its role in oral biofilm development, S. gordonii also is a pathogen in infective endocarditis in susceptible humans. A surface adhesin, Hsa, has been shown to play a critical role in colonization of S. gordonii on the heart tissue; however, its role in oral biofilm development has not been reported. In this study we demonstrate that Hsa is essential for coaggregation between S. gordonii and Veillonella sp., which are bridging species connecting the pioneer colonizers to the late colonizers. Interestingly, the same domains shown to be required for Hsa binding to sialic acid on the human cell surface are also required for coaggregation with Veillonella sp. However, sialic acid appeared not to be required for this intergeneric coaggregation. This result suggests that although the same domains of Hsa are involved in binding to eukaryotic as well as Veillonella cells, the binding mechanism is different. The gene expression pattern of hsa was also studied and shown not to be induced by coaggregation with Veillonella sp.