Heat Stress of Algal Partner Hinders Colonization Success and Alters the Algal Cell Surface Glycome in a Cnidarian-Algal Symbiosis.
Heat Stress of Algal Partner Hinders Colonization Success and Alters the Algal Cell Surface Glycome in a Cnidarian-Algal Symbiosis.
复制标题
DOI：
10.1128/spectrum.01567-22
复制
发表时间：
2022-06-29
影响因子：
3.7
通讯作者：
Weis, Virginia M.
中科院分区：
文献类型：
--
作者：
Maruyama, Shumpei;Mandelare-Ruiz, Paige E.;McCauley, Mark;Peng, Wenjing;Cho, Byeong Gwan;Wang, Junyao;Mechref, Yehia;Loesgen, Sandra;Weis, Virginia M.
关键词：
珊瑚的生态成功归功于它们与甲藻（共生藻科）的共生关系。虽然热应激对这种共生的负面影响得到了很好的研究，但热应激如何影响共生的发生和共生体特异性却很少探索。在这项工作中，我们使用的模式海葵，Exaiptasia diaphana（通常被称为Aiptasia），和它的原生共生体，Breviolum minutum，研究热胁迫对Aiptasia的藻类和藻类细胞表面糖组的殖民化的影响。热应激导致Aiptasia的藻类，不是由于混杂变量，如藻类运动或氧化应激的殖民减少。通过质谱分析和凝集素染色，鉴定了先前发现与自由生活的藻类菌株相关的聚糖（高甘露糖苷聚糖）的热诱导富集和与共生藻类菌株相关的聚糖（半乳糖基化聚糖）的伴随减少。还鉴定了特异性唾液酸聚糖的差异富集，尽管它们在这种共生中的作用仍不清楚。我们还讨论了用于分析藻类细胞表面糖组的方法，评估目前的局限性，并为藻类珊瑚糖生物学的未来工作提供建议。总的来说，这项研究提供了深入了解如何压力可能会影响刺胞动物和他们的藻类共生体之间的共生关系，通过改变糖的共生伙伴。珊瑚礁正受到全球气候变化的威胁。它们的衰落主要是由于它们与甲藻共生关系的脆弱性造成的，而甲藻是它们赖以取得生态成功的基础。为了更好地了解珊瑚生物学，研究人员使用了海葵Aiptasia，这是一种研究珊瑚-藻类共生的模型系统，并描述了热应力如何改变藻类与珊瑚宿主交流的能力。这项研究发现，热应激导致藻类定殖成功率下降，并影响藻类的细胞表面分子，使其变得更像非共生藻类物种。这项工作增加了我们对珊瑚-藻类共生所涉及的分子信号以及它在热应激期间如何分解的理解。
Corals owe their ecological success to their symbiotic relationship with dinoflagellate algae (family Symbiodiniaceae). While the negative effects of heat stress on this symbiosis are well studied, how heat stress affects the onset of symbiosis and symbiont specificity is less explored. In this work, we used the model sea anemone, Exaiptasia diaphana (commonly referred to as Aiptasia), and its native symbiont, Breviolum minutum, to study the effects of heat stress on the colonization of Aiptasia by algae and the algal cell-surface glycome. Heat stress caused a decrease in the colonization of Aiptasia by algae that were not due to confounding variables such as algal motility or oxidative stress. With mass spectrometric analysis and lectin staining, a thermally induced enrichment of glycans previously found to be associated with free-living strains of algae (high-mannoside glycans) and a concomitant reduction in glycans putatively associated with symbiotic strains of algae (galactosylated glycans) were identified. Differential enrichment of specific sialic acid glycans was also identified, although their role in this symbiosis remains unclear. We also discuss the methods used to analyze the cell-surface glycome of algae, evaluate current limitations, and provide suggestions for future work in algal-coral glycobiology. Overall, this study provided insight into how stress may affect the symbiosis between cnidarians and their algal symbionts by altering the glycome of the symbiodinian partner. IMPORTANCE Coral reefs are under threat from global climate change. Their decline is mainly caused by the fragility of their symbiotic relationship with dinoflagellate algae which they rely upon for their ecological success. To better understand coral biology, researchers used the sea anemone, Aiptasia, a model system for the study of coral-algal symbiosis, and characterized how heat stress can alter the algae's ability to communicate to the coral host. This study found that heat stress caused a decline in algal colonization success and impacted the cell surface molecules of the algae such that it became more like that of nonsymbiotic species of algae. This work adds to our understanding of the molecular signals involved in coral-algal symbiosis and how it breaks down during heat stress.
登录
查看更多内容
DOI：
10.1073/pnas.1920779117
发表时间：
2020-11-17
影响因子：
11.1
作者：
Cleves, Phillip A.;Tinoco, Amanda I.;Pringle, John R.
通讯作者：
Pringle, John R.
DOI：
10.1073/pnas.1722151115
发表时间：
2018-05-15
影响因子：
11.1
作者：
Clevesa, Phillip A.;Strader, Marie E.;Matz, Mikhail V.
通讯作者：
Matz, Mikhail V.
影响因子：
2.2
作者：
Fujise, Lisa;Nitschke, Matthew R.;Suggett, David J.
通讯作者：
Suggett, David J.
影响因子：
4.5
作者：
Lesser, MP
通讯作者：
Lesser, MP
影响因子：
4.6
作者：
Li B;Tang J;Yang Q;Cui X;Li S;Chen S;Cao Q;Xue W;Chen N;Zhu F
通讯作者：
Zhu F