Plasma Concentration of Malaria Parasite-Derived Macrophage Migration Inhibitory Factor in Uncomplicated Malaria Patients Correlates with Parasitemia and Disease Severity
Plasma Concentration of Malaria Parasite-Derived Macrophage Migration Inhibitory Factor in Uncomplicated Malaria Patients Correlates with Parasitemia and Disease Severity
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无并发症的疟疾患者中疟疾寄生虫源性巨噬细胞迁移抑制因子的血浆浓度与寄生虫血症和疾病严重程度相关
DOI：
10.1128/cvi.00149-10
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发表时间：
2010-10-01
影响因子：
--
通讯作者：
Wang, Heng
中科院分区：
文献类型：
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作者：
Han, Cong;Lin, Yahui;Wang, Heng
摘要宿主巨噬细胞移动抑制因子（MIF）参与了疟疾的发病机制。几种疟原虫衍生的MIFs被鉴定为具有调节宿主免疫应答的潜力。然而，疟原虫MIFs在疟疾感染的免疫发病机制中的作用以及这些介质与炎性细胞因子之间的关系仍不清楚。本研究对单纯性疟疾患者外周血中两种疟原虫MIFs进行了检测，并分析了其与疟疾感染过程中几个主要因素的相关性。我们发现患者体内恶性疟原虫MIF（PfMIF）和间日疟原虫MIF（PvMIF）水平与寄生虫血症、肿瘤坏死因子α、白细胞介素-10（IL-10）和单核细胞趋化蛋白1呈正相关，但与转化生长因子β1和IL-12无关。有趣的是，PvMIF水平与宿主体温和人MIF（HuMIF）浓度呈正相关。此外，多元逐步回归分析也表明，寄生虫血症，IL-10，和HuMIF表达的疟原虫MIF生产的显着预测。此外，在抗疟药物治疗期间，大多数患者的疟原虫MIF浓度降低后出现寄生虫血症。我们的研究结果表明，疟原虫MIF循环水平反映了寄生虫血症的水平，因此与疾病的严重程度密切相关，在简单的疟疾。因此，该因子有可能成为一种有前途的疾病预测因子，并可用于临床诊断。
ABSTRACT Host macrophage migration inhibitory factor (MIF) has been implicated in the pathogenesis of malaria infections. Several Plasmodium parasite-derived MIFs were identified to have the potential to regulate host immune response. However, the role of Plasmodium MIFs in the immunopathogenesis of malaria infection and the relationships between these mediators and inflammatory cytokines remained unclear. In this study, we have investigated two Plasmodium MIFs in peripheral blood of uncomplicated malaria patients and analyzed their correlations with several major factors during malaria infection. We found that both Plasmodium falciparum MIF (PfMIF) and Plasmodium vivax MIF (PvMIF) levels in patients were positively correlated with parasitemia, tumor necrosis factor alpha, interleukin-10 (IL-10), and monocyte chemoattractant protein 1 but were not correlated with transforming growth factor β1 and IL-12. Of interest was that the PvMIF level was positively correlated with host body temperature and human MIF (HuMIF) concentrations. Moreover, multiple stepwise regression analysis also showed that parasitemia, IL-10, and HuMIF expression were significant predictors of Plasmodium MIF production. In addition, during antimalarial drug treatment, the decreasing of Plasmodium MIF concentrations was followed by parasitemia in most patients. Our results suggested that the Plasmodium MIF circulating level reflects the level of parasitemia and thus was closely correlated with disease severity in uncomplicated malaria. Therefore, this factor has the potential to be a promising disease predictor and is applicable in clinical diagnosis.