The organization of the osteocyte network mirrors the extracellular matrix orientation in bone
The organization of the osteocyte network mirrors the extracellular matrix orientation in bone
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DOI：
10.1016/j.jsb.2010.11.014
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发表时间：
2011-02-01
影响因子：
3
通讯作者：
Fratzl, Peter
中科院分区：
文献类型：
--
作者：
Kerschnitzki, Michael;Wagermaier, Wolfgang;Fratzl, Peter
骨是一种动态的组织，它不断地经历着重塑的过程--这是由于破骨细胞的骨吸收和成骨细胞的骨形成之间的相互作用造成的。当新骨沉积时，一些成骨细胞嵌入矿化的胶原基质中，并分化为骨细胞，在整个骨组织中形成致密的网络。在这里，我们调查了骨细胞网络的组织在多大程度上控制了在各种骨组织中发现的胶原基质的排列。用共聚焦激光扫描显微镜、偏振光显微镜和背散射电子成像技术对马骨、羊骨和鼠骨的几种组织类型进行了研究。通过比较无组织和有组织的骨的空间排列，我们认为，高度定向的胶原基质的形成需要成骨细胞的排列，从而基质层提供一个表面，使成骨细胞能够排列并共同构建新的基质。没有这样的底物，成骨细胞就会孤立地活动，只形成没有长程有序的基质。因此，我们得出结论，成骨细胞合成并利用支架样的初级组织作为引导，通过许多细胞的集体作用来沉积高度有序和具有机械能力的骨组织。(C)2010 Elsevier Inc.保留所有权利。
Bone is a dynamic tissue that is continually undergoing a process of remodeling - an effect due to the interplay between bone resorption by osteoclasts and bone formation by osteoblasts. When new bone is deposited, some of the osteoblasts are embedded in the mineralizing collagen matrix and differentiate to osteocytes, forming a dense network throughout the whole bone tissue. Here, we investigate the extent to which the organization of the osteocyte network controls the collagen matrix arrangement found in various bone tissues. Several tissue types from equine, ovine and murine bone have been examined using confocal laser scanning microscopy as well as polarized light microscopy and back-scattered electron imaging. From comparing the spatial arrangements of unorganized and organized bone, we propose that the formation of a highly oriented collagen matrix requires an alignment of osteoblasts whereby a substrate layer provides a surface such that osteoblasts can align and, collectively, build new matrix. Without such a substrate, osteoblasts act isolated and only form matrices without long range order. Hence, we conclude that osteoblasts synthesize and utilize scaffold-like primary tissue as a guide for the deposition of highly ordered and mechanically competent bone tissue by a collective action of many cells. (C) 2010 Elsevier Inc. All rights reserved.