[Prognosis and evaluation of drug therapy by V3 and RT gene analysis of HIV-1].
[Prognosis and evaluation of drug therapy by V3 and RT gene analysis of HIV-1].
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HIV-1 V3和RT基因分析预测预后及药物治疗评价
DOI：
10.11150/kansenshogakuzasshi1970.70.347
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
M. Nakai
中科院分区：
文献类型：
--
作者：
M. Morimoto;T. Otake;H. Mori;T. Kawahata;N. Ueba;S. Okubo;K. Yasunaga;K. Sano;T. Nakano;M. Nakai
我们报道了54例HIV-1患者的分离和临床标志物的调查。我们尝试了对V3和RT基因的分析。一个迅速恶化的患者的HIV-1在V3的第11位（Arg）有碱性氨基酸，在第25位（Gln）失去酸性氨基酸。这种序列模式是一种具有快速高、合胞体诱导（SI）和t细胞系表型的病毒的显著特征。相比之下，无临床症状或轻度临床症状的患者序列特征为慢-低、非synsytium诱导（NSI）和嗜巨噬细胞。然后我们研究了AZT和ddI耐药的出现。RT基因分析表明，毒株获得耐药变异的速度比野生型快。我们认为这些关于病毒分离和基因分析的数据对预后和临床治疗策略是有用的。
We have reported on the investigation of 54 HIV-1 patients concerning the isolation and clinical marker in the preceding paper. We have attempted the analysis of the V3 and RT genes. HIV-1 from a patient who had rapidly taken a turn for the worse had basic amino acid at position 11 (Arg) and lost an acidic amino acid at position 25 (Gln) of V3. This sequence pattern was a distinguished feature of a virus with a rapid-high, syncytium inducing (SI) and T-cell-line tropic phenotype. In contrast, patients with no or mild clinical symptoms had sequences characterized as slow-low, non-synsytium inducing (NSI) and macrophage tropic. We then investigated the appearance of resistant to AZT and ddI. It was shown that the virulent virus obtained drug resistant variants faster than the wild type by analysis of the RT gene. We consider that these data concerning virus isolation and gene analysis are useful for prognosis and strategy for clinical therapy.