Lipoprotein oxidation, plasma total antioxidant capacity and homocysteine level in patients with multiple sclerosis
Lipoprotein oxidation, plasma total antioxidant capacity and homocysteine level in patients with multiple sclerosis
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DOI：
10.1080/1028415031000115945
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发表时间：
2003-06-01
影响因子：
3.6
通讯作者：
Çomoglu, S
中科院分区：
文献类型：
--
作者：
Besler, HT;Çomoglu, S
自由基介导的生物分子（尤其是脂质）的过氧化作用与多发性硬化症等许多疾病的发病机制有关。在多发性硬化症患者的血清或脑脊液中观察到低浓度的抗氧化维生素：β胡萝卜素、视黄醇、α生育酚和抗坏血酸。在此基础上，我们研究了多发性硬化发病机制中潜在的脂蛋白氧化和总抗氧化能力。本文测定了24例多发性硬化患者和24例健康人的血浆脂蛋白体外氧化能力、血清抗氧化低密度脂蛋白自身抗体、血浆总同型半胱氨酸、维生素B-12和叶酸水平及血浆总抗氧化能力。在多发性硬化症患者在攻击过程中，一个显着的增加，在体外脂质氧化的血浆和对氧化低密度脂蛋白的自身抗体的水平，和血浆总抗氧化能力的强烈下降被检测到。血浆总同型半胱氨酸水平在血浆维生素B-12和叶酸水平较低的多发性硬化患者中显著高于对照组，但无统计学意义。目前的研究表明，脂蛋白氧化可能是在多发性硬化症的过程中的重要因素，并在体外测量血浆氧化动力学作为脂蛋白氧化的指示可能是有用的作为一个额外的工具，多发性硬化症的临床诊断。
Free radical-mediated peroxidation of biological molecules, especially of lipids, is implicated in the pathogenesis of a number of diseases like multiple sclerosis. Low concentration of antioxidant vitamins: beta carotene, retinol, alpha tocopherol and ascorbic acid have been observed in serum or cerebrospinal fluid of multiple sclerosis patients. On the basis of these observations, we studied the potential lipoprotein oxidation and total antioxidant capacity in the pathogenesis of multiple sclerosis. Lipoprotein oxidizability for plasma in vitro, serum levels of autoantibodies against oxidized low-density lipoproteins, plasma total homocysteine levels with vitamin B-12 and folate, and plasma total antioxidant capacity were measured in twenty four patients with multiple sclerosis and twenty four healthy sex- and age-matched person as control. In multiple sclerosis patients during an attack, a significant increase in both in vitro lipid oxidizability for plasma and in the levels of autoantibodies against oxidized low-density lipoproteins, and a strong decrease in plasma total antioxidant capacity were detected. Plasma total homocysteine levels were significantly higher in multiple sclerosis patients whose plasma vitamin B-12 and folate levels were lower but not statistically significant, than controls. The present study indicates that lipoprotein oxidation may be important factor in the course of multiple sclerosis and in vitro measurements of plasma oxidation kinetics as an indication for lipoprotein oxidation might be useful as an additional tool for the clinical diagnosis of multiple sclerosis.