Silver accumulation in Pseudomonas stutzeri AG259.
Silver accumulation in Pseudomonas stutzeri AG259.
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DOI：
10.1007/bf01142556
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发表时间：
1989-01-01
期刊：
影响因子：
--
通讯作者：
Trevors, J T
中科院分区：
文献类型：
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作者：
Gadd, G M;Laurence, O S;Trevors, J T
银对施氏假单胞菌AG 259的毒性强烈依赖于培养基中的NaCl浓度，这降低了通过沉淀为AgCl的Ag+的可用性。在所有检查的处理中，生长培养物的银积累较低，小于或等于7.5 nmol（mg干质量）-1。生长培养基中NaCl的存在并没有显着影响银积累的细胞的量，但影响毒性表现为滞后期，这是最大的低NaCl浓度（小于或等于0.1%质量/体积）。在无NaCl培养基中，与无Ag对照相比，施氏原杆菌在0.5 mM AgNO 3存在下不生长。大多数的银积累的斯氏疟原虫的静息细胞发生在1分钟内的孵育，并有以前生长在AgNO 3的存在或不存在下的细胞之间的吸收能力的差异不大。当悬浮在含有1%（质量/体积）的1 mM Mes（pH 6.5）中时，静息细胞的Ag摄取量最低。氯化钠。施氏原杆菌暴露于Cu（NO3）2之前，悬浮在1 mM Mes pH 6.5，含有0.5 mM AgNO 3时，导致Ag吸收显著减少。
Silver toxicity to Pseudomonas stutzeri AG259 was strongly dependent on the NaCl concentration in the medium, which reduced the availability of Ag+ by precipitation as AgCl. Accumulation of Ag by growing cultures was low being less than or equal to 7.5 nmol (mg dry mass)-1 over all treatments examined. The presence of NaCl in the growth medium did not markedly affect the amounts of Ag accumulated by the cells but influenced toxicity as manifest by a lag period which was greatest at low NaCl concentrations (less than or equal to 0.1% mass/vol.). In NaCl-free medium, P. stutzeri did not grow in the presence of 0.5 mM AgNO3 in contrast to Ag-free controls. The majority of Ag accumulation by resting cells of P. stutzeri occurred within 1 min of incubation and there was little difference in uptake capacities between cells previously grown in the absence or presence of AgNO3. Lowest amounts of Ag uptake by resting cells occurred when suspended in 1 mM Mes pH 6.5, containing 1% (mass/vol.) NaCl. Prior exposure of P. stutzeri to Cu(NO3)2 resulted in a marked reduction in Ag uptake when suspended in 1 mM Mes pH 6.5, containing 0.5 mM AgNO3.