Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis
Hepatic Helicobacter species identified in bile and gallbladder tissue from Chileans with chronic cholecystitis
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DOI：
10.1016/s0016-5085(98)70589-x
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发表时间：
1998-04-01
期刊：
影响因子：
29.4
通讯作者：
Roa, I
中科院分区：
文献类型：
--
作者：
Fox, JG;Dewhirst, FE;Roa, I
背景与目标胆囊癌是智利女性癌症死亡的第一大原因。这种肿瘤的发病率在全球范围内差异很大，高危人群的发病率比低危人群高约 30 倍，这表明感染性微生物、致癌物和营养等环境因素在其发病机制中发挥了作用。因为有几种螺杆菌。幽门螺杆菌定植于动物肝脏并诱发肝炎，本研究的目的是确定幽门螺杆菌感染是否与人类胆囊炎有关。方法对 46 名接受胆囊切除术的慢性胆囊炎智利人的胆汁或切除胆囊组织进行幽门螺杆菌培养。并使用螺杆菌特异性 16S 核糖体 RNA 引物进行聚合酶链反应 (PCR) 分析。结果螺杆菌 sp. 的回收率。从冷冻标本中提取并不成功。然而，通过PCR分析，23份胆汁样本中的13份和23份胆囊组织中的9份幽门螺杆菌呈阳性。对八个螺杆菌特异性 PCR 扩增子进行了测序并进行系统发育分析。五个序列代表胆汁菌菌株、两种“Flexispira rappini”菌株 (ATCC 49317) 和一种白脓杆菌菌株。结论这些数据支持胆汁抗性螺杆菌的关联。患有胆囊疾病。需要进一步的研究来确定是否存在类似的螺杆菌。在胆囊癌的发展中发挥致病作用。胃肠病学 1998；114：755-763
Background & AimsCancer of the gallbladder is the number one cause of cancer mortality in Chilean women. Incidence rates for this tumor vary widely on a worldwide basis, being approximately 30 times higher in high-risk than in low-risk populations, suggesting that environmental factors such as infectious microorganisms, carcinogens, and nutrition play a role in its pathogenesis. Because several Helicobacter sp. colonize the livers of animals and induce hepatitis, the aim of this study was to determine whether Helicobacter infection was associated with cholecystitis in humans.MethodsBile or resected gallbladder tissue from 46 Chileans with chronic cholecystitis undergoing cholecystectomy were cultured for Helicobacter sp. and subjected to polymerase chain reaction (PCR) analysis using Helicobacter-specific 16S ribosomal RNA primers.ResultsRecovery of Helicobacter sp. from frozen specimens was unsuccessful. However, by PCR analysis, 13 of 23 bile samples and 9 of 23 gallbladder tissues were positive for Helicobacter. Eight of the Helicobacter-specific PCR amplicons were sequenced and subjected to phylogenetic analysis. Five sequences represented strains of H. bilis, two strains of “Flexispira rappini” (ATCC 49317), and one strain of H. pullorum.ConclusionsThese data support an association of bile-resistant Helicobacter sp. with gallbladder disease. Further studies are needed to ascertain whether similar Helicobacter sp. play a causative role in the development of gallbladder cancer. GASTROENTEROLOGY 1998;114:755-763