Pulau virus;: a new member of the Nelson Bay orthoreovirus species isolated from fruit bats in Malaysia
Pulau virus;: a new member of the Nelson Bay orthoreovirus species isolated from fruit bats in Malaysia
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DOI：
10.1007/s00705-005-0644-4
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发表时间：
2006-02-01
影响因子：
2.7
通讯作者：
Wang, LF
中科院分区：
文献类型：
--
作者：
Pritchard, LI;Chua, KB;Wang, LF
1998- 1999年尼帕病毒暴发导致105人死亡，并扑杀了100多万头猪。此后，在马来西亚东海岸外的Tioman岛开始寻找尼帕病毒的天然宿主宿主。从岛上的果蝠中分离出三种不同的合胞体形成病毒。它们是尼帕病毒、Tioman病毒（一种与Menangle病毒相关的新型副粘病毒）和一种名为Pulau病毒（PuV）的呼肠孤病毒，这是本研究的主题。PuV表现出呼肠孤病毒的典型超结构形态，并被血清中和，以对抗纳尔逊湾呼肠孤病毒（NBV），这是一种30多年前从澳大利亚果蝠（脊髓灰质炎翼蝠）中分离出来的呼肠孤病毒。PuV在Vero细胞中具有融合性，形成大的合胞体。PuV和NBV的dsRNA片段比较显示，S1和S2片段的迁移率存在明显差异。所有4个S片段的全序列分析表明，PuV和NBV密切相关，核苷酸序列同源性从S3片段的88%到S1片段的56%不等。对推断出的蛋白序列进行类似的系统发育分析，证实PuV与NBV密切相关。本文讨论了PuV病毒与NBV病毒的异同点，这些异同点支持PuV病毒属于呼肠孤病毒科纳尔逊湾正呼肠孤病毒属的一种新型哺乳动物，融合性呼肠孤病毒的分类。
After the outbreak of Nipah virus (NiV) in 1998-99, which resulted in 105 human deaths and the culling of more than one million pigs, a search was initiated for the natural host reservoir of NiV on Tioman Island off the east coast of Malaysia. Three different syncytia-forming viruses were isolated from fruit bats on the island. They were Nipah virus, Tioman virus (a novel paramyxovirus related to Menangle virus), and a reovirus, named Pulau virus (PuV), which is the subject of this study. PuV displayed the typical ultra structural morphology of a reovirus and was neutralised by serum against Nelson Bay reovirus (NBV), a reovirus isolated from a fruit bat (Pteropus poliocephalus) in Australia over 30 years ago. PuV was fusogenic and formed large syncytia in Vero cells. Comparison of dsRNA segments between PuV and NBV showed distinct mobility differences for the S1 and S2 segments. Complete sequence analysis of all four S segments revealed a close relationship between PuV and NBV, with nucleotide sequence identity varying from 88% for S3 segment to 56% for the S1 segment. Similarly phylogenetic analysis of deduced protein sequences confirmed that PuV is closely related to NBV. In this paper we discuss the similarities and differences between PuV and NBV which support the classification of PuV as a novel mammalian, fusogenic reovirus within the Nelson Bay orthoreovirus species, in the genus Orthoreovirus, family Reoviridae.