Retinoic acid derived from the fetal ovary initiates meiosis in mouse germ cells
Retinoic acid derived from the fetal ovary initiates meiosis in mouse germ cells
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DOI：
10.1002/jcp.24172
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发表时间：
2013-03-01
影响因子：
5.6
通讯作者：
Xia, Guoliang
中科院分区：
文献类型：
--
作者：
Mu, Xinyi;Wen, Jing;Xia, Guoliang
生殖细胞减数分裂起始于13.5？DPC（交配后天数）表示小鼠中的雌性性别决定。近年来的研究表明，中肾源视黄酸（RA）是诱导减数分裂的关键信号。然而，中肾是否为减数分裂所必需尚不清楚，子房在减数分裂过程中的作用也有待澄清。本研究提供的数据表明，来自胎儿卵巢的RA足以在胎儿卵巢培养系统中诱导生殖细胞减数分裂。在11.5 ~ 13.5岁时采集胎儿卵巢;分离并体外培养的DPC胎儿，生殖细胞在没有中肾的情况下进入减数分裂。排除RA来源于中肾，11.5？dpc尿生殖嵴（UGR;用二乙基氨基苯甲醛（DEAB）处理中肾和卵巢复合体（mesonephroi and ovary complex）以阻断中肾和卵巢中的视黄醇脱氢酶（RALDH）活性。当DEAB退出时发生减数分裂，2天后取出中肾。此外，RALDH 1，而不是RALDH 2，作为主要的RA合成酶在UGR从12.5至15.5？民主党。DEAB单独处理卵巢能够阻断生殖细胞减数分裂进入。我们还发现，外源性RA剂量依赖性地减少卵巢生殖细胞的数量，加速进入减数分裂。这些结果表明，卵巢来源的RA是负责减数分裂启动。J.细胞。228：627639，2013。(C)2012 Wiley Periodicals，Inc.
Meiotic initiation of germ cells at 13.5?dpc (days post-coitus) indicates female sex determination in mice. Recent studies reveal that mesonephroi-derived retinoic acid (RA) is the key signal for induction of meiosis. However, whether the mesonephroi is dispensable for meiosis is unclear and the role of the ovary in this meiotic process remains to be clarified. This study provides data that RA derived from fetal ovaries is sufficient to induce germ cell meiosis in a fetal ovary culture system. When fetal ovaries were collected from 11.5 to 13.5?dpc fetuses, isolated and cultured in vitro, germ cells enter meiosis in the absence of mesonephroi. To exclude RA sourcing from mesonephroi, 11.5?dpc urogenital ridges (UGRs; mesonephroi and ovary complexes) were treated with diethylaminobenzaldehyde (DEAB) to block retinaldehyde dehydrogenase (RALDH) activity in the mesonephros and the ovary. Meiosis occurred when DEAB was withdrawn and the mesonephros was removed 2 days later. Furthermore, RALDH1, rather than RALDH2, serves as the major RA synthetase in UGRs from 12.5 to 15.5?dpc. DEAB treatment to the ovary alone was able to block germ cell meiotic entry. We also found that exogenously supplied RA dose-dependently reduced germ cell numbers in ovaries by accelerating the entry into meiosis. These results suggest that ovary-derived RA is responsible for meiosis initiation. J. Cell. Physiol. 228: 627639, 2013. (C) 2012 Wiley Periodicals, Inc.