The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice.
The "Ly-1 B" cell subpopulation in normal immunodefective, and autoimmune mice.
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DOI：
10.1084/jem.157.1.202
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
Herzenberg LA
中科院分区：
文献类型：
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作者：
Hayakawa K;Hardy RR;Parks DR;Herzenberg LA
携带所有经典B细胞标志物（IgM、IgD、Ia和ThB）的正常鼠脾B细胞的小亚群也携带主要T细胞表面分子之一Ly-1。该“Ly-1 B”亚群（通过多参数FACS分析鉴定和表征）由相对较大的高IgM/低IgD/低Ly-1淋巴细胞组成，其代表正常动物脾细胞的约2%，并且通常代表新西兰B小鼠脾细胞的5-10%。在所有测试的正常小鼠品系以及NZ B、CBA/N、其它X-id小鼠和裸（nu/nu）小鼠中可清楚地检测到Ly-1 B。它们主要存在于脾脏中;在淋巴结、骨髓和胸腺中不存在或很少出现;在个体发育早期出现，约占5天龄小鼠少量淋巴细胞的三分之一。NZ B和（NZ B x NZW）F1小鼠比所有其他品系具有更多的Ly-1 B，此外，还具有独特的Ly-1 B群体，当在不添加抗原的情况下在通常条件下培养时，该群体会分泌IgM。这些Ly-1 B细胞的IgM分泌解释了先前报道的培养物中NZ B脾细胞的“自发”IgM分泌。用FACS分选的细胞进行的研究表明，这些IgM分泌细胞上Ly-1的存在将它们与用绵羊红细胞刺激后在NZB小鼠中产生的（Ly-1阴性）IgM分泌“直接”空斑形成细胞区分开来。
A small subpopulation of normal murine splenic B cells carrying all of the classic B cells markers (IgM, IgD, Ia, and ThB) also carries Ly-1, one of the major T cell surface molecules. This "Ly-1 B" subpopulation (identified and characterized by multiparameter FACS analyses) consists of relatively large, high IgM/low-IgD/low-Ly-1 lymphocytes that represent approximately 2% of the spleen cells in normal animals and, generally, 5-10% of spleen cells in NZB mice. Ly-1 B are clearly detectable in all normal mouse strains tested as well as NZB, CBA/N, other X-id mice and nude (nu/nu) mice. They are found primarily in the spleen; are either absent or very poorly represented in lymph node, bone marrow, and thymus; appear early during ontogeny, and comprise about a third of the small number of lymphocytes present in 5-d-old mice. NZB and (NZB x NZW)F1 mice have more Ly-1 B than all other strains and, furthermore, have a unique Ly-1 B population that secretes IgM when cultured under usual conditions in the absence of added antigen. The IgM secretion by these Ly-1 B cells accounts for the previously reported "spontaneous" IgM secretion by NZB spleen cells in culture. Studies with FACS-sorted cells show that the presence of Ly-1 on these IgM-secreting cells distinguishes them from the (Ly-1 negative) IgM-secreting "direct" plaque-forming cells generated in NZB mice after stimulation with sheep erythrocytes.