Destructive effects of butyrate on the cell envelope of Helicobacter pylori
Destructive effects of butyrate on the cell envelope of Helicobacter pylori
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DOI：
10.1099/jmm.0.039040-0
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发表时间：
2012-04-01
影响因子：
3
通讯作者：
Kamiya, Shigeru
中科院分区：
文献类型：
--
作者：
Yonezawa, Hideo;Osaki, Takako;Kamiya, Shigeru
幽门螺杆菌存在于口腔中，主要通过 PCR 技术检测。幽门螺杆菌的生长受到口腔内多种因素的影响，如口腔微生物群、唾液和其他抗菌物质，所有这些都使得幽门螺杆菌难以在口腔定植。在本研究中，我们分析了牙周代表性细菌牙龈卟啉单胞菌的细胞上清液对幽门螺杆菌的影响，发现细胞上清液破坏了幽门螺杆菌细胞包膜。由于牙龈卟啉单胞菌产生丁酸，我们将研究重点放在丁酸盐的作用上，发现它显着抑制幽门螺杆菌的生长。丁酸盐处理后，在细胞外环境中检测到幽门螺杆菌细胞质蛋白和DNA，这表明细胞包膜的完整性受到损害，表明丁酸盐对幽门螺杆菌具有杀菌作用。此外，用产丁酸细菌的细胞上清液处理后，细胞外幽门螺杆菌DNA的水平增加，表明细胞上清液也具有杀菌作用，这可能是由于其丁酸含量所致。总之，产生丁酸的细菌可能在影响幽门螺杆菌在口腔定植中发挥作用。
Helicobacter pylori can be found in the oral cavity and is mostly detected by the use of PCR techniques. Growth of H. pylori is influenced by various factors in the mouth, such as the oral microflora, saliva and other antimicrobial substances, all of which make colonization of the oral cavity by H. pylori difficult. In the present study, we analysed the effect of the cell supernatant of a representative periodontal bacterium Porphyromonas gingivalis on H. pylori and found that the cell supernatant destroyed the H. pylori cell envelope. As P. gingivalis produces butyric acid, we focused our research on the effects of butyrate and found that it significantly inhibited the growth of H. pylori. H. pylori cytoplasmic proteins and DNA were detected in the extracellular environment after treatment with butyrate, suggesting that the integrity of the cell envelope was compromised and indicating that butyrate has a bactericidal effect on H. pylori. In addition, levels of extracellular H. pylori DNA increased following treatment with the cell supernatant of butyric acid-producing bacteria, indicating that the cell supernatant also has a bactericidal effect and that this may be due to its butyric acid content. In conclusion, butyric acid-producing bacteria may play a role in affecting H. pylori colonization of the oral cavity.