Genetic analysis of pertussis toxin promoter activation in Bordetella pertussis.
Genetic analysis of pertussis toxin promoter activation in Bordetella pertussis.
复制标题
百日咳博德特氏菌中百日咳毒素启动子激活的遗传分析。
DOI：
10.1046/j.1365-2958.1997.4371792.x
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发表时间：
1997
影响因子：
3.6
通讯作者：
Carbonetti,NH
中科院分区：
文献类型：
--
作者：
Marques,RR;Carbonetti,NH
Bordetella pertussisregulates expression of its virulence factors such as pertussis toxin (Ptx) via thebvglocus, which encodes a two‐component system composed of a sensor protein, BvgS, and a transcription activator, BvgA. We used aptx–lacfusion on theB. pertussischromosome to analyse promoter activation by alteration of specific sequences upstream of and within the promoter. Our data demonstrate that a pair of heptanucleotide inverted repeats separated by a turn of the DNA helix within the upstream repeat region (centred around nucleotide −136.5) are crucialcis‐activating elements, and probably represent the initial BvgA‐binding site. In addition, we demonstrate that the sequence between these repeats and the promoter plays a role in activation. Our data are most consistent with a model of co‐operative binding of BvgA dimers to this intervening region and interaction with RNA polymerase at the promoter to activateptxtranscription. In the core promoter region both the non‐consensus 21 bp spacing and the specific sequence between the −35 and −10 elements are crucial for promoter activity.
