Evaluation of 5 µm Superficially Porous Particles for Capillary and Microfluidic LC Columns.
Evaluation of 5 µm Superficially Porous Particles for Capillary and Microfluidic LC Columns.
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评估5 µM毛细血管和微流体LC色谱柱的表面多孔颗粒。
DOI：
10.3390/chromatography2030502
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发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Kennedy RT
中科院分区：
文献类型：
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作者：
Grinias JP;Kennedy RT
当填充到大孔径色谱柱中时，大尺寸（4-5 µm）表面多孔颗粒比类似尺寸的全多孔颗粒产生更低的塔板高度（例如，最小减少的塔板高度或 hmin ≈ 1.5）。此特性可实现更好的色谱性能，而无需较小颗粒所需的更高压力。本研究探讨了此类颗粒在微流控液相色谱柱中的使用，其中材料和安装压力限制可能会限制所用颗粒的尺寸。与全多孔颗粒相比，理论上预测的性能改进并未在毛细管柱中得到证实（两种颗粒类型的 hmin ≈ 2），这与之前检查较小表面多孔颗粒的研究一致。然后将微流体柱与毛细管柱进行比较。由于微流体通道不对称和最佳流速下的世界与芯片连接带来的缺陷，毛细管柱的性能显着优于微流体柱；然而，填充在微流控液相色谱柱中的表面多孔颗粒具有更平坦的板高度与流速曲线，表明在高降低速度下具有更好性能的潜力。
Large-size (4–5 µm) superficially porous particles yield lower plate heights (e.g., the minimal reduced plate height or hmin ≈ 1.5) than fully porous particles of a similar size when packed into large-bore columns. This property allows for better chromatographic performance without the higher pressures required for smaller particles. This study explores the use of such particles in microfluidic LC columns where materials and fitting pressure limits can constrain the size of particle used. The theoretically predicted performance improvements compared to fully porous particles were not demonstrated in capillary columns (with hmin ≈ 2 for both particle types), in agreement with previous studies that examined smaller superficially porous particles. Microfluidic columns were then compared to capillary columns. Capillary columns significantly outperformed microfluidic columns due to imperfections imposed by microfluidic channel asymmetry and world-to-chip connection at the optimal flow rate; however, superficially porous particles packed in microfluidic LC columns had flatter plate height versus flow rate curves indicating potential for better performance at high reduced velocities.