Parasitic infections and immune function: Effect of helminth infections in a malaria endemic area
Parasitic infections and immune function: Effect of helminth infections in a malaria endemic area
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DOI：
10.1016/j.imbio.2012.08.273
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发表时间：
2013-05-01
期刊：
影响因子：
2.8
通讯作者：
Eriksson, Ulrika K.
中科院分区：
文献类型：
--
作者：
Boef, Anna G. C.;May, Linda;Eriksson, Ulrika K.
根据卫生假说，减少感染可以解释高收入国家特应性疾病的增加。假设蠕虫改变宿主的免疫应答以避免消除，并且因此也降低宿主对潜在过敏原的应答。为了阐明当前蠕虫感染对人类免疫反应的影响，我们测量了加纳农村人口中的细胞因子产生，该地区有多种地方性寄生虫，包括疟疾，肠道蠕虫和原生动物。多重实时PCR检测粪便样本中的四个胃肠道蠕虫，其中只有美洲钩虫是常见的。一个类似的检测方法被用来检测粪便样本中的贾第虫和静脉血样本中的疟疾感染。在用脂多糖（LPS）和酵母聚糖（用于先天性细胞因子产生）或T细胞有丝分裂原植物血凝素（PHA）体外刺激的全血样品中测定细胞因子白细胞介素（IL）-10、肿瘤坏死因子（TNE）-α、IL-17、IL-6、IL-13和干扰素（IFN）-γ的水平。取决于当前N的先天或PHA刺激的细胞因子产生没有显着差异。美洲感染。恶性疟原虫疟疾感染与促炎反应相关，其表现为TNF-α、IL-17和IL-6的先天产生增加。依赖于G的细胞因子反应没有明确的模式。兰比拉感染总之，在加纳农村人口目前N。美洲疟原虫感染与免疫功能改变无关，而恶性疟原虫感染与促炎性先天免疫应答有关。(C)2012 Elsevier GmbH. All rights reserved.
According to the hygiene hypothesis, reduced exposure to infections could explain the rise of atopic diseases in high-income countries. Helminths are hypothesised to alter the host's immune response in order to avoid elimination and, as a consequence, also reduce the host responsiveness to potential allergens. To elucidate the effect of current helminth infections on immune responsiveness in humans, we measured cytokine production in a rural Ghanaian population in an area with multiple endemic parasites including malaria, intestinal helminths and protozoa. Multiplex real-time PCR in stool samples was used for the detection of four gastrointestinal helminths, of which only Necator americanus was commonly present. A similar assay was used to test for Giardia lamblia in stool samples and malaria infection in venous blood samples. Levels of the cytokines interleukin (IL)-10, tumour necrosis factor (TNE)-alpha, IL-17, IL-6, IL-13, and interferon (IFN)-gamma were determined in whole-blood samples ex vivo-stimulated either with lipopolysaccharide (LPS) and zymosan (for innate cytokine production) or the T-cell mitogen phytohaemagglutinin (PHA). There were no significant differences in either innate or PHA-stimulated cytokine production dependent on current N. americanus infection. Plasmodium falciparum malarial infection was associated with a pro-inflammatory response indicated by increased innate production of TNF-alpha, IL-17 and IL-6. There was no clear pattern in cytokine responses dependent on G. lambila-infection. In conclusion, in this rural Ghanaian population current N. americanus infections are not associated with altered immune function, while infection with P. falciparum is associated with pro-inflammatory innate immune responses. (C) 2012 Elsevier GmbH. All rights reserved.