Visualization of nutrient translocation in ectomycorrhizal symbioses.
Visualization of nutrient translocation in ectomycorrhizal symbioses.
复制标题
外生菌根共生体中养分易位的可视化。
作者：
Miyashita K;他;Dan xu;Miura N. & Asano Y;Wu B
外生菌根真菌(ECM)从寄主树接受光合作用固定的碳，并从土壤中提供磷(P)和氮(N)等养分。在一个二维无土根箱中建立了一个ECM共生系统，利用成像平板的数字时程放射自显影技术来可视化ECM共生过程中的养分转移。用~(14)C和~(33)P放射性同位素示踪实验，讨论了~(33)P-磷酸和光合作用固定碳在真菌与寄主树木之间以及菌丝体之间通过菌丝连接而转移的几项研究。此外，还讨论了可以可视化菌根共生期间养分转移的新技术。
Ectomycorrhizal (ECM) fungi receive photosynthetically fixed carbon from the host tree and, in return, supply nutrients such as phosphorus (P) and nitrogen (N) from the soil. An ECM symbiosis system in a two-dimensional, soil-free rhizobox was developed to visualize nutrient translocation during ECM symbioses using a digital, time-course autoradiographic technique with imaging plates. Several studies using14C and33P radioisotope tracing experiments are discussed to demonstrate the translocation of33P-phosphoric acid and photosynthetically fixed carbon between fungi and host trees and between mycelia via mycelia anastomosis. Additionally, novel techniques that can visualize nutrient translocation during mycorrhizal symbioses are discussed.