Ultrastructure of ceramic-bone interface using hydroxyapatite and β-tricalcium phosphate ceramics and replacement mechanism of β-tricalcium phosphate in bone
Ultrastructure of ceramic-bone interface using hydroxyapatite and β-tricalcium phosphate ceramics and replacement mechanism of β-tricalcium phosphate in bone
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DOI：
10.1016/s0040-8166(03)00067-3
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发表时间：
2003-12-01
期刊：
影响因子：
2.6
通讯作者：
Kawasaki, T
中科院分区：
文献类型：
--
作者：
Fujita, R;Yokoyama, A;Kawasaki, T
羟基磷灰石（HA）和β-磷酸三钙（β-TCP）可用于骨组织的移植和增强。本研究通过透射电子显微镜（TEM）观察生物材料与邻近组织之间界面的超微结构以及生物材料周围的成骨作用。将HA和β-TCP陶瓷制成具有大孔和微孔的圆盘形式，植入大鼠的顶骨和颅骨骨膜之间。术后4周和8周制备标本进行观察。显微镜结果显示HA表面存在中间层，而β-TCP表面不存在中间层。在 HCl 脱钙作用下，HA 和骨之间的中间层观察到特征性纤维状结构。在β-TCP中，通过光学显微镜观察到靠近骨组织的网状结构中，钙化和稀疏的胶原纤维散布在β-TCP颗粒之间。此外，靠近β-TCP和骨之间的界面处，存在许多具有众多突起的骨细胞。这些结果揭示了HA与β-TCP界面超微结构的差异以及β-TCP在骨中的溶解机制。 (C) 2003 Elsevier Ltd. 保留所有权利。
Hydroxyapatite (HA) and beta-tricalcium phosphate (beta-TCP) are useful for grafting and augmentation of bone tissue. Observation by transmission electron microscopy (TEM) was done to investigate the ultrastructures at the interfaces between the biomaterials and the adjacent tissue, and osteogenesis around the biomaterials in the present study. HA and beta-TCP ceramics were used in disk forms which had macropores and micropores, and were implanted between the parietal bone and the cranial periosteum of rats. Specimens were prepared for observation at 4 and 8 weeks postoperatively.The microscopic results indicated that an intervening layer was present on the surface of HA, whereas it was not present on the surface of beta-TCP. A characteristic fibrillar structure was observed in the intervening layer between HA and bone under decalcification by HCl. In beta-TCP, in reticular structures observed close to the bone tissue by optical microscopy, calcification and sparse collagen fibers were interspersed among the granules of beta-TCP. In addition, close to the interface between beta-TCP and bone, many osteocytes with numerous processes were present. Some processes were elongated towards the inter-face.These results revealed the difference in the ultrastructures of the inter-faces between HA and beta-TCP, and the dissolution mechanism of beta-TCP in bone. (C) 2003 Elsevier Ltd. All rights reserved.