Expression analysis of phytohormone-regulated microRNAs in rice, implying their regulation roles in plant hormone signaling

Expression analysis of phytohormone-regulated microRNAs in rice, implying their regulation roles in plant hormone signaling
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水稻中植物激素调节的 microRNA 的表达分析,表明它们在植物激素信号传导中的调节作用

DOI:
10.1016/j.febslet.2009.01.020
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发表时间:
2009-02-18
期刊:
影响因子:
3.5
通讯作者:
Chen, Yue-Qin
Chen, Yue-Qin
中科院分区:
生物学3区
文献类型:
--
作者:
Liu, Qing;Zhang, Yu-Chan;Chen, Yue-Qin

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选择22个保守的miRNAs研究植物激素处理对水稻基因表达的影响,并分析了5种典型的植物激素胁迫对水稻基因表达的影响。结果表明,11个miRNAs被发现由一个或多个植物激素处理失调。这些miRNAs的靶基因在体内被验证,并且它们的表达促进了细胞的增殖。林透露。我们还分析了22个保守的miRNAs的启动子区的植物激素响应元件,这些元件的存在为我们的结果提供了进一步的证据。这些发现使我们能够进一步研究miRNAs在植物激素信号传导中的作用。(C)2009年欧洲生物化学学会联合会。Elsevier B.V.出版,保留所有权利。
Twenty-two conserved miRNAs were chosen to investigate the expression pattern in response to phytohormone treatments, in which the effects of five classic plant hormone stresses were surveyed in Oryza sativa. The results showed that 11 miRNAs were found to be dysregulated by one or more phytohormone treatments. The target genes of these miRNAs were validated in vivo and their expression pro. ling were revealed. We also analyzed the promoter regions of the 22 conserved miRNAs for phytohormone-responsive elements and the existence of the elements provided further evidences supporting our results. These findings enable us to further investigate the role of miRNAs in phytohormone signaling. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.