Instability of the protoplast membrane of facultative alkaliphilic Bacillus sp. C-125 at alkaline pH values below the pH optimum for growth.
Instability of the protoplast membrane of facultative alkaliphilic Bacillus sp. C-125 at alkaline pH values below the pH optimum for growth.
复制标题
兼性嗜碱性芽孢杆菌原生质体膜的不稳定性。
DOI：
10.1042/bj2850099
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Koki Horikoshi
中科院分区：
文献类型：
--
作者：
Rikizo Aono;Masahiro Ito;Koki Horikoshi
兼性嗜碱芽孢杆菌C-125的细胞壁由三种聚合物组成（肽聚糖、铁醛酸和铁醛酸）。用蛋白溶菌酶制备的原生质体在中性pH下可再生细胞壁，但在pH高于8.5时不能再生细胞壁。原生质体在碱性条件下易发生裂解。原生质体在碱性条件下迅速破裂，失去细胞壁再生能力。碱稳定性与嗜中性枯草芽孢杆菌（Bacillus subtilis）原生质体相似。在碱性pH下，膜囊泡也不稳定。菌株C-125的酸性壁成分可能有助于在碱性pH下生长的细胞的细胞质膜的稳定，可能是通过屏蔽膜直接暴露于碱性环境。
Cell walls of facultative alkaliphilic Bacillus sp. C-125 consist of three polymers (peptidoglycan, teichuronopeptide and teichuronic acid). Protoplasts prepared from the strain with egg-white lysozyme regenerated cell walls at neutral pH, but not at pH above 8.5. The protoplasts were susceptible to lysis at alkaline pH. The protoplasts exposed to alkaline pH rapidly burst and lost ability to regenerate their cell walls. The alkali-instability was similar to that of protoplasts from neutrophilic Bacillus subtilis 168. The membrane vesicles were also labile at alkaline pH. The acidic wall components of strain C-125 may contribute to stabilization of the cytoplasmic membrane of cells growing at alkaline pH, probably by shielding the membrane from direct exposure to an alkaline environment.