Enzymatic fluorometric procedure for phospholipid quantification with an automated microtiter plate fluorometer.
Enzymatic fluorometric procedure for phospholipid quantification with an automated microtiter plate fluorometer.
复制标题
使用自动微量滴定板荧光计进行磷脂定量的酶荧光测定程序。
DOI：
10.1093/clinchem/37.6.868
复制
发表时间：
1991
影响因子：
9.3
通讯作者：
N. Miller
中科院分区：
文献类型：
--
作者：
M. Nanjee;A. Gebre;N. Miller
We describe a new enzymatic assay for phospholipids that is rapid, sensitive, convenient, and inexpensive. The method is based on the fluorometric detection of H2O2, generated by the choline oxidase-catalyzed oxidation of choline, after liberation of choline from phospholipids by phospholipase D. Significant advantages over existing methods are that the entire reaction sequence can take place in a single vessel, a 12 x 8 well microtiter plate, and that the fluorescence intensity can be measured automatically with a Fluoroskan II (Labsystems Oy) detector. Extracting samples with organic solvents is unnecessary, although the method can be applied to extracts in isopropanol. The assay is approximately 60-fold more sensitive and has a limit of detection eightfold lower than currently available enzymatic colorimetric methods. Including solvent blanks, eight standards, and three quality-control pools, 34 samples can be pipetted and assayed in duplicate in 60 min. Results obtained by this procedure for total phospholipid choline in lipoproteins in primate plasma agreed well with those obtained for inorganic phosphorus by the Bartlett acid-digestion procedure.
影响因子：
--
作者：
Edelstein,C;Scanu,AM
通讯作者：
Scanu,AM