Difference in the expression of CIRBP, RBM3 and HSP70 in the myocardium and cerebellum after death by hypothermia and carbon monoxide poisoning
Difference in the expression of CIRBP, RBM3 and HSP70 in the myocardium and cerebellum after death by hypothermia and carbon monoxide poisoning
复制标题
低温和一氧化碳中毒死亡后心肌和小脑CIRBP、RBM3和HSP70表达的差异
DOI：
10.4323/rjlm.2013.219
复制
发表时间：
2013
影响因子：
0.4
通讯作者：
K. Nishi
中科院分区：
文献类型：
--
作者：
Satomu Morita;S. Furukawa;Lisa Wingenfeld;Wakoto Matsuda;K. Nishi
我们研究了缺氧相关抗原的表达（例如，冷诱导抗原和凋亡抗原）的心肌和小脑，从一氧化碳或低温死亡后的个人获得。免疫组化结果显示，冷诱导RNA结合蛋白（CIRBP）和RNA结合蛋白3（RBM3）的表达可能与高温和低氧条件有关。CIRBP和RBM3在心肌中的表达与在小脑中的表达不同，尤其是在浦肯野细胞中。结果表明，濒死持续时间影响抗原表达。在低温条件下，心肌使用更多的ATP，因为当实验温度从35 ° C降低到25 ° C时，心肌的兴奋-收缩偶联力增加了400%以上。本研究结果提示临床医师在对患者进行降温时，应注意心肌保护，以保护脑组织。关键词：一氧化碳死亡，小脑，CIRBP，低温死亡，心肌，RBM3。
We studied the expression of hypoxia-related antigens (e.g., cold-inducible antigens and apoptotic antigens) in the myocardium and the cerebellumthat were obtained from individuals after death by carbon monoxide or hypothermia. The immunohistochemistry results revealed that expression of cold-inducible RNA binding protein (CIRBP) and RNA-binding protein 3 (RBM3) may be associated with hpyothermic and the hypoxic conditions. The expression of CIRBP and RBM3 in the myocardium was different from their expression in the cerebellum, especially in the Purkinje cells. The results indicate that agonal duration influences antigen expression. In the hypothermic condition, the myocardium uses more ATP since the force of the excitation-contraction coupling of the myocardium increases by more than 400% when the experimental temperature is reduced from 35°C to 25°C. The results obtained in this study indicate that physicians should pay attention to the myocardium when cooling the patient's body to protect the brain. Key W ords: carbon monoxide death, cerebellum, CIRBP, hypothermic death, myocardium, RBM3.