Differential and inefficient splicing of a broadly expressed Drosophila erect wing transcript results in tissue-specific enrichment of the vital EWG protein isoform.
Differential and inefficient splicing of a broadly expressed Drosophila erect wing transcript results in tissue-specific enrichment of the vital EWG protein isoform.
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广泛表达的果蝇直立翼转录本的差异和低效剪接导致重要的 EWG 蛋白亚型的组织特异性富集。
DOI：
10.1128/mcb.19.6.3998
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发表时间：
1999
影响因子：
5.3
通讯作者：
White,K
中科院分区：
文献类型：
--
作者：
Koushika,SP;Soller,M;DeSimone,SM;Daub,DM;White,K
在这份报告中，我们记录了一种不寻常的模式，组织富集的基因表达，主要是介导的选择性和低效剪接。我们分析了果蝇直立翼基因的转录后调节，该基因提供了重要的神经元功能，并且对于某些肌肉的形成至关重要。其主要蛋白质产物，116-kDa EWG蛋白，一种推定的转录调节因子，可以提供所有已知的与翅膀相关的功能。此外，与其功能一致，116-kDa蛋白在神经元中高度富集，并且在迁移的成肌细胞中也观察到短暂的。与蛋白质分布相反，我们观察到，在成年果蝇富含神经元的头部和缺乏神经元的身体中，直翅转录本的水平相当。我们的分析表明，该rectwingtranscript由10个外显子组成，并选择性剪接，内含子的子集是无效剪接。我们还表明，116 kDa的EWG蛋白编码剪接异构体的头部富集。相比之下，身体有较低水平的转录本，可以编码116 kDa的蛋白质和大量的未经加工的直接wingRNA。因此，116-kDa蛋白在头部的富集是通过组织特异性选择性和低效剪接而不是通过转录调控来确保的。此外，这种调节在生物学上是重要的，因为神经系统外116-kDa蛋白水平的增加是致命的。
In this report, we document an unusual mode of tissue-enriched gene expression that is primarily mediated by alternative and inefficient splicing. We have analyzed posttranscriptional regulation of theDrosophila erect winggene, which provides a vital neuronal function and is essential for the formation of certain muscles. Its predominant protein product, the 116-kDa EWG protein, a putative transcriptional regulator, can provide all knownerect wing-associated functions. Moreover, consistent with its function, the 116-kDa protein is highly enriched in neurons and is also observed transiently in migrating myoblasts. In contrast to the protein distribution, we observed thaterect wingtranscripts are present in comparable levels in neuron-enriched heads and neuron-poor bodies of adultDrosophila. Our analyses shows thaterect wingtranscript consists of 10 exons and is alternatively spliced and that a subset of introns are inefficiently spliced. We also show that the 116-kDa EWG protein-encoding splice isoform is head enriched. In contrast, bodies have lower levels of transcripts that can encode the 116-kDa protein and greater amounts of unprocessederect wingRNA. Thus, the enrichment of the 116-kDa protein in heads is ensured by tissue-specific alternative and inefficient splicing and not by transcriptional regulation. Furthermore, this regulation is biologically important, as an increased level of the 116-kDa protein outside the nervous system is lethal.