Parathyroid Hormone in Human Plasma: IMMUNOCHEMICAL CHARACTERIZATION AND BIOLOGICAL IMPLICATIONS.
Parathyroid Hormone in Human Plasma: IMMUNOCHEMICAL CHARACTERIZATION AND BIOLOGICAL IMPLICATIONS.
复制标题
人血浆中的甲状旁腺激素：免疫化学特征和生物学意义。
DOI：
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发表时间：
1972
影响因子：
15.9
通讯作者：
J. Potts
中科院分区：
文献类型：
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作者：
G. Segre;J. Habener;D. Powell;G. Tregear;J. Potts
四种抗牛甲状旁腺激素抗血清的抗原识别特征在于它们与牛激素片段（1-34、1-13、14-34、19-34、53-84）和从甲状旁腺腺瘤提取的人激素的反应性。所有的抗血清被发现有抗体群体，识别一个以上的抗原决定簇，所有的抗血清不同的特异性和反应性的牛激素的片段。通过用过量浓度的1-34或53-84片段预孵育修饰两种抗血清GP-1和GP-133，抗原识别被限制在激素序列的确定区域。使用特异性测定氨基甲状旁腺激素的测定，在牛和人甲状旁腺激素之间观察到更大的免疫化学相似性，当使用这些抗血清的测定应用于研究人血浆中的内源性甲状旁腺激素时，显示出在全身循环中的免疫反应性激素基本上缺乏完整激素序列的氨基末端部分，包括需要全部或部分14-19区的抗原决定簇。这种删除帐户，至少部分地，为血浆甲状旁腺激素在man. Radioimmunoassay进行凝胶过滤的外周血浆馏分的免疫化学异质性证实，在一般循环的人的免疫反应性激素的主要形式是一个激素片段，是完全没有氨基末端的反应。由于这一缺失，可以得出结论，大多数免疫反应性甲状旁腺激素在人体的一般循环必须是生物活性。
Antigenic recognition of four anti-bovine parathyroid hormone antisera was characterized by their reactivity with bovine hormonal fragments (1-34, 1-13, 14-34, 19-34, 53-84) and human hormone extracted from parathyroid adenomas. All antisera were found to have antibody populations which recognized more than one antigenic determinant and all antisera differed in their specificity and reactivity for the fragments of bovine hormone. By modification of two antisera, GP-1 and GP-133, by preincubation with excess concentrations of 1-34 or 53-84 fragments, antigenic recognition was restricted to defined regions of the hormonal sequence.When assays using these modified antisera were applied to the study of hormones extracted from glands, greater immunochemical similarities were seen between bovine and human parathyroid hormone using assays that were specific for the measurement of amino-terminal portions of the hormones than of the carboxy-terminal portions.When assays using these antisera were applied to the study of endogenous parathyroid hormone in human plasma, the immunoreactive hormone in the general circulation was shown to substantially lack an amino-terminal portion of the sequence of the intact hormone, including an antigenic determinant requiring all or some of the 14-19 region. This deletion accounts, at least in part, for the immunochemical heterogeneity of plasma parathyroid hormone in man. Radioimmunoassay of fractions of peripheral plasma subjected to gel filtration confirms that the dominant form of the immunoreactive hormone in the general circulation of man is a hormonal fragment that is totally devoid of amino-terminal reactivity. Because of this deletion, it can be concluded that most of the immunoreactive parathyroid hormone in the general circulation of man must be biologically inactive.