Synchronous fluorescence measurement of BaP metabolites in human and animal urine
Synchronous fluorescence measurement of BaP metabolites in human and animal urine
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DOI：
10.1080/00032718708062926
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发表时间：
1987-05
影响因子：
2
通讯作者：
M. Uziel;R. Ward;T. Vo‐Dinh
中科院分区：
文献类型：
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作者：
M. Uziel;R. Ward;T. Vo‐Dinh
大鼠体内注射苯并(a)芘可引起尿中BaP荧光代谢物的排泄。同步荧光（SF）技术可以检测到7、8、9、10-四氢-四羟基苯并(a)芘（BPT）等荧光化合物的皮图量。以BPT为模型化合物，SF可通过简单稀释检测尿中0.1μM的多核芳香族化合物（PNA）或PNA代谢物。相对较高的浓度限值是由于需要将尿液稀释100倍才能观察到特征芘谱。由于尿猝灭作用，不稀释全尿直接定量BPT的上限为5μM。将尿液稀释100倍，添加BPT，恢复正常荧光和SF光谱。0.1 μM的检测限低于未暴露、不吸烟人群中观察到的单个PNA的量。这种方法可能为监测人体暴露于荧光有毒物质提供一种简单、无创的工具。
Abstract Injection of Benzo(a)pyrene into rats results in urinary excretion of fluorescent metabolites of BaP. The synchronous fluorescence (SF) technique can detect picogram quantities of fluorescent compounds such as 7,8,9,10-tetrahydro-tetrahydroxy-benzo(a)pyrene (BPT). BPT was used as a model compound to show that SF can detect 0.1μM of polynuclear aromatic compounds (PNA) or PNA metabolites in urine by simple dilution. The relatively high concentration limit is due to the need to dilute the urine 100 fold before the characteristic pyrenyl spectrum is observed. The upper limit of direct quantification of BPT without dilution of whole urine is 5μM because of urine quenching effects. A 100-fold dilution of urine, containing added BPT, restores normal fluorescence and SF spectra. The 0.1 μM detection limit is lower than the observed amounts of individual PNA in nonexposed, nonsmoking humans. This approach may provide a simple, noninvasive tool for monitoring human exposure to fluorescent toxic substances.