The serum proteomics tracking of hepatocellular carcinoma early recurrence following radical resection
The serum proteomics tracking of hepatocellular carcinoma early recurrence following radical resection
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肝细胞癌根治术后早期复发的血清蛋白质组学追踪。
DOI：
10.2147/cmar.s190561
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发表时间：
2019-01-01
影响因子：
3.3
通讯作者：
Liu, Jingfeng
中科院分区：
文献类型：
--
作者：
Liu, Hongzhi;Chen, Hui;Liu, Jingfeng
目的：目前尚缺乏预测肝细胞癌早期复发的高度敏感和特异的生物标志物，这阻碍了临床预后的进一步改善。我们的目标是寻找高度敏感和特异的生物标志物来预测肝细胞癌复发。患者和方法：采用基于相对和绝对定量等压标记(ITRAQ)的多维液相色谱-串联质谱仪(2D LC-MS/MS)技术，对4例肝细胞癌根治术后患者术后血清蛋白质组变化进行了定量研究和监测。结果：共鉴定出27个与肝癌早期复发密切相关的差异丰富蛋白(DAP)，这些DAP主要集中在ERK1/2和核因子-κβ信号通路中，提示这两条信号通路的异常在肝癌早期复发的病理过程中起重要作用。对一组患者的进一步调查证实，血清中高水平的PGK1与肝癌的早期复发和预后不良密切相关。此外，血清PGK1水平可与AFP相互补充，进一步提高预测肝癌复发的敏感性和特异性。结论：PGK1可能是影响肝癌复发的独立因素。PGK1可与AFP互补，进一步提高预测肝癌复发的敏感性和特异性。
Purpose: There is still lacking of highly sensitive and specific biomarkers for the prediction of hepatocellular carcinoma (HCC) early recurrence, which has hindered further improvement of the clinical outcomes. We aim to find highly sensitive and specific biomarkers for the prediction of HCC recurrence. Patients and methods: By using isobaric tags for relative and absolute quantitation (iTRAQ)-based multidimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) technique, we have quantitatively investigated and monitored the proteome alterations of a series of serum after radical resection during the follow-up of 4 HCC patients. Results: A total of 27 differentially abundant proteins (DAPs) in serum were identified to be closely associated with the early recurrence of HCC, and these DAPs were particularly concentrated within ERK1/2 and nuclear factor-κ beta signaling pathways, suggesting the dysregulation of these two pathways played an important role in the pathological process of HCC early recurrence. Further investigation of a cohort of patients confirmed that the high serum level of PGK1 was closely associated with HCC early recurrence and poor prognosis. In addition, the serum level of PGK1 could be complementary with AFP to further improve the sensitivity and specificity for predicting the relapse of HCC. Conclusion: PGK1 might be an independent factor for the recurrence of HCC. And the PGK1 could be complementary with AFP to further improve the sensitivity and specificity in prognostic prediction of HCC relapse.