Protective Effect of Garlic on Cellular Senescence in UVB-Exposed HaCaT Human Keratinocytes.
Protective Effect of Garlic on Cellular Senescence in UVB-Exposed HaCaT Human Keratinocytes.
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DOI：
10.3390/nu8080464
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发表时间：
2016-07-29
期刊：
影响因子：
5.9
通讯作者：
Kim HK
中科院分区：
文献类型：
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作者：
Kim HK
紫外线（UV）照射引起细胞内产生活性氧（ROS），从而导致细胞衰老和光老化。本研究探讨了大蒜对UVB照射的人角质形成细胞（HaCaT细胞）的光损伤和细胞衰老的保护作用。采用体外无细胞体系，测定其清除DPPH自由基和一氧化氮（NO）的活性。观察大蒜提取物对UVB照射HaCaT细胞后ROS生成、MMP-1蛋白和mRNA表达、细胞因子如白细胞介素（IL）-1β和IL-6、衰老相关β-半乳糖苷酶（SA-β-gal）活性和沉默信息调节因子T1（SIRT 1）活性的影响。大蒜对DPPH自由基和NO有较强的清除作用，IC_（50）分别为2.50 mg/mL和4.38 mg/mL。大蒜预处理减弱UVB诱导的细胞内ROS的产生。MMP-1水平，这已经被称为是由活性氧诱导，显着提高UVB照射，和UVB诱导的MMP-1的mRNA和蛋白质表达显着降低大蒜处理（50微克/毫升）相比，紫外线未暴露的控制细胞。大蒜预处理可显著抑制UV诱导的促炎细胞因子（IL-6和IL-1β）的产生，且呈剂量依赖性。SA-β-gal活性，细胞衰老的经典生物标志物，和SIRT 1活性，近年来作为抗衰老因子引起了人们的关注，通过大蒜处理在UV照射的HaCaT细胞中得到改善。本研究提供了大蒜抑制UVB诱导的光老化的第一个证据，作为HaCaT人角质形成细胞衰老的结果。
Ultraviolet (UV) irradiation generates reactive oxygen species (ROS) in the cells, which induces the cellular senescence and photoaging. The present study investigated the protective effects of garlic on photo-damage and cellular senescence in UVB-exposed human keratinocytes, HaCaT cells. An in vitro cell free system was used to examine the scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals and nitric oxide (NO). The effect of garlic extract on ROS formation, MMP-1 protein and mRNA expressions, cytokines such as interleukin (IL)-1β and IL-6, senescence associated-β-galactosidase (SA-β-gal) activity, and silent information regulator T1 (SIRT1) activity were determined in UVB-irradiated HaCaT cells. Garlic exhibited strong DPPH radical and NO scavenging activity in cell free system exhibiting IC50 values of 2.50 mg/mL and 4.38 mg/mL, respectively. Garlic pretreatment attenuated the production of UVB-induced intracellular ROS. MMP-1 level, which has been known to be induced by ROS, was dramatically elevated by UVB irradiation, and UVB-induced MMP-1 mRNA and protein expressions were significantly reduced by garlic treatment (50 µg/mL) comparable to those of UV-unexposed control cells. UV-induced pro-inflammatory cytokine productions (IL-6 and IL-1β) were significantly inhibited by pretreatment with garlic in a dose-dependent manner. SA-β-gal activity, a classical biomarker of cellular senescence, and SIRT1 activity, which has attracted attention as an anti-aging factor in recent years, were ameliorated by garlic treatment in UV-irradiated HaCaT cells. The present study provides the first evidence of garlic inhibiting UVB-induced photoaging as a result of augmentation of cellular senescence in HaCaT human keratinocytes.